Antibody immobilization on functional monolayers using a quartz crystal microbalance
Antibody immobilization on functional monolayers using a quartz crystal microbalance
复制标题
DOI：
10.1109/jsen.2006.881368
复制
发表时间：
2006-10-01
影响因子：
4.3
通讯作者：
Kurosawa, Shigeru
中科院分区：
文献类型：
--
作者：
Aizawa, Hidenobu;Gokita, Yasutoshi;Kurosawa, Shigeru
研究了2-氨基乙硫醇（AET）、4,4′-二硫代二丁酸（DDA）和11-巯基癸酸（MUA）在石英晶体微天平（QCM）金表面沉积时，抗c -反应蛋白（CRP）单克隆抗体在石英晶体微天平上的固定化效果。在所有的单层中，抗crp抗体被固定，如Langmuir型，因为它已经引入了相应的活性基团。根据Langmuir等温线方程a(max)，抗crp抗体的最大固定化量分别为4.27、2.72和3.74 pmol/cm(2)。虽然抗CRP抗体在AET单层上的固定量最高，但抗CRP抗体与CRP之间的抗原抗体结合量在MUA单层上最高。当使用MUA单层获得校准曲线时，CRP从阴性水平检测到阳性水平。
This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation a(max), the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm(2), respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.