Molecular analysis of Chinese truffles resembling Tuber californicum in morphology reveals a rich pattern of species diversity with emphasis on four new species
Molecular analysis of Chinese truffles resembling Tuber californicum in morphology reveals a rich pattern of species diversity with emphasis on four new species
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DOI：
10.3852/14-343
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发表时间：
2016-03-01
期刊：
影响因子：
2.8
通讯作者：
Yan, Xiang-yuan
中科院分区：
文献类型：
--
作者：
Fan, Li;Han, Ling;Yan, Xiang-yuan
在中国，种名Tuber californicum被分配给具有或多或少规则球形子囊孢子的Tuber属中的浅色松露;然而，我们最近发现该名称被错误地应用于在中国采集的一些标本。在本研究中，我们重新审查了所有中国收集据称是T。包括植物标本和新采集的材料，并与形态学和分子生物学方法。根据这些材料的内转录间隔区序列进行系统发育分析，将这些材料分为7个类群。生成ML树。通过ITS、nuc 28 S rDNA和翻译延伸因子-1 α序列分析，中国和世界各地的球形子囊孢子种仅分为两个分支，即芽囊菌（Puberulum Glade）和孢子囊菌（Acutosporum Glade）。在这项研究中所认识到的孢草包括已知仅来自东亚的物种，并可能代表了一个新的谱系，与青春期，斑点和Gibbosum谱系平行出现。我们的结论是没有证据支持T. californicum的新记录，误认为是T californicum的标本属于4个新记录种，即T. xuanhuaense、T. jinshajiangense、T. caoi和T.小木藓属
In China the species name Tuber californicum is assigned to pale truffles in the genus Tuber that have more or less regularly globose ascospores; however, we recently discovered that the name was mis-applied to some specimens collected in China. In the present study we re-examined all available Chinese collections purported to be T. californicum, including herbarium specimens and newly collected materials, with morphological and molecular methods. The accessions separated into seven clusters in a phylogenetic analysis based on sequences of the internal transcribed spacers sequences of these materials. An ML tree generated. with ITS, nuc 28S rDNA and translation elongation factor-1 alpha sequences from Chinese and worldwide Tuber species with globose ascospores showed only two clades, the Puberulum Glade and Latisporum Glade. The Latisporum Glade as recognized in this study includes the species known from eastern Asia only and probably represents a new lineage that arose in parallel with the Puberulum, Maculatum and Gibbosum lineages. We concluded that no evidence supports the existence of T. californicum in China and that the specimens misidentified as T californicum belong to four species newly described herein, T. xuanhuaense, T. jinshajiangense, T. caoi and T. parvomurphium.