Inhibitory effect of mitragynine, an analgesic alkaloid from Thai herbal medicine, on neurogenic contraction of the vas deferens
Inhibitory effect of mitragynine, an analgesic alkaloid from Thai herbal medicine, on neurogenic contraction of the vas deferens
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DOI：
10.1016/j.lfs.2005.04.042
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发表时间：
2005-11-26
期刊：
影响因子：
6.1
通讯作者：
Horie, S
中科院分区：
文献类型：
--
作者：
Matsumoto, K;Yamamoto, LT;Horie, S
从泰国草药Kratom（Mitragyna speciosa）中分离的吲哚生物碱mitragynine对豚鼠输精管平滑肌神经源性收缩的影响进行了研究。Mitragynine抑制电刺激引起的输精管收缩。另一方面，mitragynine不影响对去甲肾上腺素和ATP的反应。在河豚毒素、哌唑嗪和α，β-亚甲基ATP存在下，Mitragynine不降低KCl诱导的收缩。Mitragynine抑制尼古丁或酪胺诱导的收缩。采用膜片钳技术，发现mitragynine可阻断神经母细胞瘤细胞N1 E-115的T型和L型钙通道电流。在通过荧光染料法的Ca 2+测量中，mitragynine减少KCl诱导的神经母细胞瘤细胞中的Ca 2+内流。本研究结果提示，mitragynine抑制输精管收缩引起的神经刺激，可能是通过其阻断神经元Ca ~（2+）通道。(c)2005年爱思唯尔公司All rights reserved.
The effect of an indole-alkaloid mitragynine isolated from the Thai medicinal herb kratom (Mitragyna speciosa) on neurogenic contraction of smooth muscle was studied in guinea-pig vas deferens. Mitragynine inhibited the contraction of the vas deferens produced by electrical transmural stimulation. On the other hand, mitragynine failed to affect the responses to norepinephrine and ATP. Mitragynine did not reduce KCl-induced contraction in the presence of tetrodotoxin, prazosin and alpha,beta-methylene ATP. Mitragynine inhibited nicotine- or tyramine-induced contraction. By using the patch-clamp technique, mitragynine was found to block T- and L-type Ca2+ channel currents in N1E-115 neuroblastoma cells. In the Ca2+ measurement by a fluorescent dye method, mitragynine reduced KCl-induced Ca2+ influx in neuroblastoma cells. The present results suggest that mitragynine inhibits the vas deferens contraction elicited by nerve stimulation, probably through its blockade of neuronal Ca2+ channels. (c) 2005 Elsevier Inc. All rights reserved.