DIFFERENTIAL REGULATION OF WAXY GENE-EXPRESSION IN RICE ENDOSPERM
DIFFERENTIAL REGULATION OF WAXY GENE-EXPRESSION IN RICE ENDOSPERM
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DOI：
10.1007/bf00254822
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
SANO, Y
中科院分区：
文献类型：
--
作者：
SANO, Y
为了研究不同等位基因对蜡质基因表达的影响，从水稻胚乳淀粉中分离了控制直链淀粉合成的Wx基因产物，并进行了电泳分析。与淀粉粒结合的主要蛋白质在大多数蜡质品系中缺失，并随着三倍体胚乳中Wx等位基因数目的增加而增加，表明主要蛋白质是Wx基因产物。除了导致Wx基因产物和直链淀粉缺失或急剧减少的Wx等位基因外，Wx等位基因似乎在非糯稻品系中也发生了分化。至少检测到2个对主要蛋白质和直链淀粉产生不同效率的Wx等位基因。这些等位基因与基因表达的调节有关。
In order to examine the effects of different alleles on the gene expression at the waxy locus, the Wx gene product which controls the synthesis of amylose was isolated from endosperm starch of rice plants [Oryza sativa and O. glaberrima strains] and analyzed by electrophoretic techniques. The major protein bound to starch granules was absent in most of waxy strains and increased with the number of Wx alleles in triploid endosperms, suggesting that the major protein is the Wx gene product. In addition to wx alleles which result in the absence or drastic reduction of the Wx gene product and amylose, differentiation of Wx alleles seemed to have occurred among nonwaxy rice strains. At least 2 Wx alleles with different efficiencies in the production of the major protein and amylose were detected. These alleles are discussed in relation to regulation of the gene expression.