High Dietary Calcium and Vitamin D Effects on Fat Mass Accretion and Expression of Liver Enzymes in Rats
High Dietary Calcium and Vitamin D Effects on Fat Mass Accretion and Expression of Liver Enzymes in Rats
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高膳食钙和维生素 D 对大鼠脂肪量增加和肝酶表达的影响
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
D. Teegarden
中科院分区：
文献类型：
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作者：
Shamim M K Siddiqui;Eugene Chang;Mi Zou;S. Koser;K. Buhman;S. Donkin;D. Teegarden
高钙 (Ca) 和维生素 D (D) 摄入量可能会减少脂肪量 (FM) 的堆积。我们研究了高钙和高维生素 D 摄入量对身体成分、底物氧化 (Ox) 和相关肝酶基因表达的影响。雄性 Wistar 大鼠（n = 32，200 gms）喂食高脂肪（HF，40％）或高蔗糖（HS，60％）饮食，其中含有低水平（LoDCa）Ca（0.25％）和D（400 IU）或高水平（HiDCa）Ca（1.5％）和D（10,000 IU），持续14周。 FM 和去脂体重 (LBM) 通过 DEXA 测定。在肝脏和肌肉组织匀浆中测量 14C-棕榈酸酯和 14C-葡萄糖 Ox。通过实时 RT PCR 评估磷酸烯醇丙酮酸羧基激酶 (PEPCK)、脂肪酸合酶和肉碱棕榈酰转移酶 1 基因表达。 HiDCa 组与 LoDCa 组或 HF 组与 HS 组的采食量或体重增加没有差异。与 LoDCa 相比，无论 HF 或 HS，HiDCa 饮食组的 FM 较低（73%，P<0.05），LBM 较高（106%，P<0.05）。仅用 HF 饮食喂养 HiDCa 的大鼠的肝脏葡萄糖 Ox 降低（P<0.05）。 Ca 或 D 摄入量对肝酶的表达没有影响。与 HS 饮食相比，HF 饮食中 PEPCK 的基因表达较高（P<0.05）。因此，HiDCa 饮食可减少脂肪堆积并增加 LBM，与饮食能量来源无关。 HiDCa 改变了 HF 肝脏中的底物氧化，但 HS 饮食则不然，表明这不是 HiDCa 调节身体成分的作用机制。
High calcium (Ca) and vitamin D (D) intakes may lead to reduced fat mass (FM) accretion. We examined the effects of high Ca and D intake on body composition, substrate oxidation (Ox) and gene expression of related liver enzymes. Male Wistar rats (n=32, 200 gms) were fed high fat (HF, 40%) or high sucrose (HS, 60%) diets with either low levels (LoDCa) of Ca (0.25%) and D (400 IU) or high levels (HiDCa) of Ca (1.5%) and D (10,000 IU) for 14 weeks. FM and lean body mass (LBM) were determined by DEXA. 14C‐palmitate and 14C‐glucose Ox were measured in liver and muscle tissue homogenates. Phosphoenolpyruvate carboxy kinase (PEPCK), fatty acid synthase, and carnitine palmitoyl transferase‐1 gene expression was assessed by real time RT PCR. There were no differences in feed intake or weight gain for HiDCa vs. LoDCa or HF vs. HS groups. FM was lower (73%, P<0.05) and LBM higher (106%, P<0.05) with HiDCa diet compared to LoDCa, regardless of HF or HS. Liver glucose Ox was decreased (P<0.05) for rats fed HiDCa only with HF diets. There was no effect of Ca or D intake on expression of liver enzymes. Gene expression of PEPCK was higher in HF diets as compared to HS diets (P<0.05). Thus, HiDCa diet reduces fat accretion and increases LBM, independent of dietary energy source. HiDCa altered substrate oxidation in liver with HF, but not with HS diets, suggesting this is not the mechanism of action of HiDCa in modulating body composition.