REPAIR AND REORGANIZATION OF MINCED CARDIAC-MUSCLE IN ADULT NEWT (NOTOPHTHALMUS-VIRIDESCENS)
REPAIR AND REORGANIZATION OF MINCED CARDIAC-MUSCLE IN ADULT NEWT (NOTOPHTHALMUS-VIRIDESCENS)
复制标题
DOI：
10.1002/jmor.1051550307
复制
发表时间：
1978-01-01
影响因子：
1.5
通讯作者：
OBERPRILLER, JO
中科院分区：
文献类型：
--
作者：
BADER, D;OBERPRILLER, JO
对成年哺乳动物和两栖动物脑室损伤反应的研究表明，在受伤或截肢的肌肉处形成结缔组织疤痕。与哺乳动物的肌细胞不同，靠近伤口表面的两栖动物肌细胞具有增殖能力。在本研究中，将切碎的心肌移植物放入成年蝾螈[N。为了增加伤口表面附近肌细胞的数量，病毒下降[心室]。随着反应性肌细胞数量的增加，人们认为主要由肌肉组成的新壁可能会形成。十六分之一到八分之一的心室尖被切除，切碎，并回到心室的截肢面。假手术动物2只，实验动物5只，分别于术后5、10、20、30、50、70天处死。碎心肌修复和重组的主要事件包括血凝块的形成，随后是血凝块和大部分移植物的坏死。到第10天，观察到明显的肌肉碎片合并和心室腔和移植物腔的连续性，尽管移植物区域从未与损伤的心室壁形成一个完整的单元。移植细胞有丝分裂活性（3.19%）和胸腺嘧啶标记（28.1%）在第20天达到峰值，其中许多细胞与心肌细胞相似。在第30天，观察到移植物主要由肌纤维组成的连续壁。一些30、50和70天的移植物有节律性收缩。两栖动物心肌显然具有哺乳动物心肌所没有的组织发生和增殖能力。
Studies of the response of adult mammalian and amphibian ventricle to injury have indicated the formation of a connective tissue scar in the place of the wounded or amputated muscle. Amphibian myocytes adjacent to a wound surface, unlike mammalian myocytes, have a proliferative capacity. In the present study, a minced cardiac muscle graft was placed into the adult newt [N. viridescens] ventricle in order to increase the number of myocytes near a wound surface. With such an increased number of reactive myocytes, it was thought a new wall consisting primarily of muscle might be formed. One-sixteenth to one-eighth of the ventricular apex was removed, minced and returned to the amputation surface of the ventricle. General histological and autoradiographic studies were conducted on 2 sham-operated animals and on 5 experimental animals which were killed at 5, 10, 20, 30, 50 and 70 days after surgery. Major events of the repair and reorganization of minced cardiac muscle included blood clot formation followed by necrosis of the blood clot and much of the muscle graft. By 10 days, an apparent coalescence of muscle fragments and continuity of ventricular and graft lumina were observed, although the graft area never formed an integrated unit with the wounded ventricular wall. The peak of mitotic activity (3.19%) and thymidine labeling (28.1%) of graft cells, including many cells which resembled cardiac myocytes, was observed at 20 days. At 30 days, the graft was observed as a continuous wall composed primarily of muscle fibers. Several 30-, 50- and 70-day grafts had rhythmic contractions. Amphibian cardiac muscle apparently has histogenetic and proliferative capacities not attributable to mammalian cardiac muscle.