In vivo expression of IL-12 and IL-13 in atopic dermatitis
In vivo expression of IL-12 and IL-13 in atopic dermatitis
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DOI：
10.1016/s0091-6749(96)70246-4
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发表时间：
1996-07-01
影响因子：
14.2
通讯作者：
Leung, DYM
中科院分区：
文献类型：
--
作者：
Hamid, Q;Naseer, T;Leung, DYM
特应性皮炎（AD）的既往研究表明，急性和慢性皮肤病变与TH 2型细胞因子表达谱相关。IL-12和IL-13是最近描述的细胞因子，其分别具有TH 1样和TH 2样作用。我们已经使用原位杂交技术，以研究IL-12和IL-13信使RNA的表达在皮肤活检标本的急性和慢性皮肤病变和未涉及的皮肤从AD患者。当与正常对照皮肤相比时，来自AD患者的急性和慢性皮肤病变以及未受影响的裙部具有显著更大数量的IL-13 mRNA阳性细胞（p < 0.05）。急性AD皮损中IL-12 mRNA阳性细胞数高于慢性AD皮损（p < 0.05）或银屑病皮损（p < 0.05）。慢性AD皮损中IL-12 mRNA阳性细胞数显著高于急性AD皮损和AD患者未受累皮肤（p < 0.05）。这些数据表明，急性AD皮肤病变与IL-13 mRNA的表达增加有关。相比之下，IL-12 mRNA在慢性AD皮肤病变中的相对增加表明IL-12产生细胞在调节慢性炎症中的可能作用。
Previous studies in atopic dermatitis (AD) have shown that acute and chronic skin lesions are associated with a TH2-type profile of cytokine expression. IL-12 and IL-13 are recently described cytokines, which possess TH1- and TH2- like actions, respectively. We have used the technique of in situ hybridization to examine the expression of IL-12 and IL-13 messenger RNA in skin biopsy specimens of acute and chronic skin lesions and uninvolved skin from patients with AD. When compared with normal control skin, the acute and chronic skin lesions and unaffected skirt from patients with AD had significantly greater numbers of cells that were positive for IL-13 mRNA (p < 0.05). Acute AD skin lesions expressed a higher number of positive cells than those observed in chronic AD skin lesions (p < 0.05) or psoriasis skin lesions (p < 0.05) There was a significant increase in the numbers of IL-12 mRNA-positive cells in chronic skin lesions compared with acute lesions and uninvolved skin from patients with AD (p < 0.05). These data demonstrate that acute AD skin lesions are associated with an increased expression of IL-13 mRNA. In contrast, the relative increase in IL-12 mRNA in chronic AD skin lesions suggests a possible role for IL-12-producing cells in modulating chronic inflammation.