Inactivation of O6-alkylguanine-DNA alkyltransferase in HeLa cells by cisplatin.
Inactivation of O6-alkylguanine-DNA alkyltransferase in HeLa cells by cisplatin.
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顺铂使 HeLa 细胞中的 O6-烷基鸟嘌呤-DNA 烷基转移酶失活。
DOI：
10.1093/carcin/10.9.1681
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发表时间：
1989
期刊：
影响因子：
4.7
通讯作者：
R. B. Setlow
中科院分区：
文献类型：
--
作者：
Long;R. B. Setlow
研究了顺铂对HeLa CCL 2细胞中O 6-烷基鸟嘌呤-DNA烷基转移酶（O 6-AGT）的失活作用。用顺铂处理的HeLa CCL 2细胞显示O 6-AGT活性的剂量依赖性下降。在去除顺铂并用新鲜培养基替换后，转移酶水平开始缓慢上升。到72小时，恢复了略高于80%的活性。似乎烷基转移酶的活性可以被铂化DNA加合物灭活。这些数据表明，O 6-铂-鸟嘌呤形成和O 6-烷基转移酶消耗不负责细胞毒性，但可能导致哺乳动物细胞中的碱基取代突变。
Inactivation of O6-alkylguanine-DNA alkyltransferase (O6-AGT) in HeLa CCL2 cells by cisplatin was studied. HeLa CCL2 cells treated with cisplatin showed a dose-dependent decline in O6-AGT activity. After cisplatin was removed and replaced with fresh medium, the transferase level began to rise slowly. By 72 h slightly more than 80% of the activity was recovered. It seems that the activity of the alkyltransferase can be inactivated by platinated DNA adducts. The data suggest that the O6-platinum-guanine formation and the O6-alkyltransferase depletion are not responsible for cytotoxicity but may result in a base substitution mutation in mammalian cells.