Immunolocalization of peroxisome proliferator-activated receptors and retinoid X receptors in the adult rat CNS
Immunolocalization of peroxisome proliferator-activated receptors and retinoid X receptors in the adult rat CNS
复制标题
DOI：
10.1016/j.neuroscience.2003.08.064
复制
发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Cerù, MP
中科院分区：
文献类型：
--
作者：
Moreno, S;Farioli-Vecchioli, S;Cerù, MP
过氧化物酶体增殖物激活和维甲酸X受体（PPARs和RXRs）是属于类固醇激素受体超家族的转录因子。在被其配体激活后，PPARs和RXR以异二聚体的形式结合至其靶基因。这些受体的配体包括亲脂性分子，如类维生素A、脂肪酸和类花生酸，它们在神经组织的代谢和功能中的重要性已被充分证明。所有的过氧化物酶体增殖物激活受体（α，β/δ和γ）和RXR（α，β和γ）同种型进行检测，并发现表现出特定的模式定位在不同的区域的大脑和脊髓。在神经元和神经胶质细胞中均观察到核受体的存在。虽然PPAR β/δ和RXR β显示出广泛的分布，但α和γ同种型表现出更有限的表达模式。额叶皮质，基底神经节，网状结构，一些脑神经核，小脑深核，小脑高尔基体细胞出现相当丰富的所有研究受体。根据我们的数据，我们认为，在成年中枢神经系统中，PPARs和RXRs，除了发挥许多其他组织的共同作用，可能有特定的功能，在调节参与神经传递的基因的表达，因此发挥作用的复杂过程，如老化，神经退行性变，学习和记忆。(C)2003年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Peroxisome proliferator-activated and retinoid X receptors (PPARs and RXRs) are transcription factors belonging to the steroid hormone receptor superfamily. Upon activation by their ligands, PPARs and RXRs bind to their target genes as heterodimers. Ligands of these receptors include lipophylic molecules, such as retinoids, fatty acids and eicosanoids, the importance of which in the metabolism and functioning of the nervous tissue is well documented.The immunohistochemical distribution of PPARs and RXRs in the CNS of the adult rat was studied by means of a sensitive biotinyl-tyramide method. All PPAR (alpha, beta/delta and gamma) and RXR (alpha, beta and gamma) isotypes were detected and found to exhibit specific patterns of localization in the different areas of the brain and spinal cord. The presence of the nuclear receptors was observed in both neuronal and glial cells. While PPAR beta/delta and RXR beta showed a widespread distribution, alpha and gamma isotypes exhibited a more restricted pattern of expression. The frontal cortex, basal ganglia, reticular formation, some cranial nerve nuclei, deep cerebellar nuclei, and cerebellar Golgi cells appeared rather rich in all studied receptors. Based on our data, we suggest that in the adult CNS, PPARs and RXRs, besides playing roles common to many other tissues, may have specific functions in regulating the expression of genes involved in neurotransmission, and therefore play roles in complex processes, such as aging, neurodegeneration, learning and memory. (C) 2003 IBRO. Published by Elsevier Ltd. All rights reserved.