CA2+ OSCILLATIONS IN PANCREATIC-ISLET CELLS SECRETING GLUCAGON AND SOMATOSTATIN
CA2+ OSCILLATIONS IN PANCREATIC-ISLET CELLS SECRETING GLUCAGON AND SOMATOSTATIN
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DOI：
10.1006/bbrc.1995.1387
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发表时间：
1995-03-17
影响因子：
3.1
通讯作者：
HELLMAN, B
中科院分区：
文献类型：
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作者：
BERTS, A;GYLFE, E;HELLMAN, B
免疫组织化学鉴定的小鼠胰岛释放胰高血糖素的 α(2) 细胞在 3 mM 葡萄糖中表现出细胞质 Ca2+ 浓度的大幅度振荡。在 20 mM 葡萄糖存在下具有类似振荡的其他小胰岛细胞被鉴定为释放生长抑素的 α(1) 细胞。两种细胞类型的振荡类似于周围较大β细胞中葡萄糖诱导的振荡，从基础水平开始，并在添加电压依赖性Ca2+通道阻滞剂甲氧基维拉帕米后消失。 α(1) 和 α(2) 细胞具有产生振荡 Ca2+ 信号的内在能力，这一发现表明生长抑素和胰高血糖素的脉冲式释放不需要与 β 细胞功能耦合。 (C) 1995 年学术出版社。公司
Immunohistochemically identified glucagon-releasing alpha(2)-cells from mouse pancreatic islets exhibited large amplitude oscillations of the cytoplasmic Ca2+ concentration in 3 mM glucose. Other small islet cells with similar oscillations in the presence of 20 mM glucose were identified as somatostatin-releasing alpha(1)-cells. The oscillations in both cell types resembled those induced by glucose in the surrounding larger beta-cells in starting from the basal level and disappearing after addition of the voltage-dependent Ca2+ channel blocker methoxyverapamil. The discovery that the alpha(1)- and alpha(2)-cells have intrinsic abilities to generate oscillatory Ca2+ signals indicates that pulsatile release of somatostatin and glucagon do not require functional coupling to the beta-cells. (C) 1995 Academic Press. Inc.