A rapid-curing alginate gel system: utility in periosteum-derived cartilage tissue engineering
A rapid-curing alginate gel system: utility in periosteum-derived cartilage tissue engineering
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DOI：
10.1016/j.biomaterials.2003.07.002
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发表时间：
2004-02-01
期刊：
影响因子：
14
通讯作者：
Shastri, VP
中科院分区：
文献类型：
--
作者：
Stevens, MM;Qanadilo, HF;Shastri, VP
在这项研究中，我们开发了一种快速固化的藻酸盐凝胶系统，并证明了其作为关节软骨组织工程应用中骨膜衍生软骨形成支架的实用性。通过用75 mM CaCl 2溶液诱导高G含量藻酸盐（65- 75%G）的2%（w/v）溶液的凝胶化来配制均匀的机械稳定的凝胶。该凝胶在低水平的变形（15%，R-2 = 0.996）下表现出近弹性行为，杨氏模量为0.17+/-0.01MPa，并且具有快速的胶凝动力学（< 1分钟完成）。软骨细胞在凝胶中的体外细胞培养产生藻酸盐/细胞结构，其缺乏透明软骨的连续的、互连的胶原蛋白/蛋白聚糖网络。此外，我们已经证明，这种凝胶系统能够支持骨膜衍生的软骨形成。我们观察到，当骨膜的全组织外植体在凝胶内体外培养时，6周后，骨膜外植体的总面积的显著量（> 50%）由软骨组成，其外观为透明样并含有软骨特异性蛋白聚糖和II型胶原。可以设想，这种外植体可以在生物可降解的藻酸盐基质内体内移植或再生，用于治疗关节软骨中的部分或全层缺损。重要的是，凝胶的注射递送可用于通过微创手术填充关节面中的复杂缺损。(C)2003年由Elsevier Ltd.出版
in this study, we have developed a rapid-curing alginate gel system and demonstrated its utility as a scaffold for periosteum-derived chondrogenesis for articular cartilage tissue engineering applications. A homogeneous mechanically stable gel was formulated by inducing gelation of a 2% (w/v) solution of a high G content alginate (65-75% G) with a 75mM solution of CaCl2. The gel exhibited near-elastic behavior at low levels of deformation (15%, R-2 = 0.996), Young's modulus of 0.17+/-0.01 MPa, and rapid gelation kinetics (< 1 min to completion). The in vitro cell culture of chondrocytes in the gel yielded alginate/cell constructs that lacked the continuous, interconnected collagen/proteoglycan network of hyaline cartilage. In addition, we have demonstrated that this gel system is capable of supporting periosteum-derived chondrogenesis. We observed that when whole-tissue explants of periosteum were cultured in vitro within the gel, after 6 weeks, significant quantities ( > 50%) of the total area of the periosteal explants was composed of cartilage that was hyaline-like in appearance and contained cartilage-specific proteoglycans and type-II collagen. It is envisioned that such explants could be transplanted or regenerated in vivo within the biodegradable alginate matrix for the treatment of partial or full-thickness defects in articular cartilage. Importantly, the injectable delivery of the gel could be used in filling complex defects in the articular surface via minimally invasive procedures. (C) 2003 Published by Elsevier Ltd.