Chemical approaches for profiling dynamic palmitoylation.
Chemical approaches for profiling dynamic palmitoylation.
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DOI：
10.1042/bst20120271
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发表时间：
2013-02-01
影响因子：
3.9
通讯作者：
Martin BR
中科院分区：
文献类型：
--
作者：
Martin BR
蛋白质棕榈酰化是一种关键的翻译后修饰，对许多关键信号蛋白的膜区划、运输和调控非常重要。最近的非放射性化学蛋白质组标记方法使人们能够将新的重点放在这一新的调节修饰上。棕榈酰化蛋白质现在可以在复杂的生物系统中通过质谱学进行直接注释和定量。基于这些分析，棕榈酰化显然是广泛存在的，并广泛影响许多细胞通路的功能。最近引入的选择性化学标记方法为重新审视关于这种广泛的翻译后修饰的酶调节的长期存在的问题打开了新的机会。在这篇综述中，我们将讨论新的化学标记方法的影响和未来对蛋白质棕榈酰化的动态全球分析的挑战。
Protein palmitoylation is a critical post-translational modification important for membrane compartmentalization, trafficking, and regulation of many key signaling proteins. Recent non-radioactive chemo-proteomic labeling methods have enabled a new focus on this emerging regulatory modification. Palmitoylated proteins can now be profiled in complex biological systems by mass spectrometry for direct annotation and quantification. Based on these analyses, palmitoylation is clearly widespread and broadly influences the function of many cellular pathways. The recent introduction of selective chemical labeling approaches has opened new opportunities to revisit long-held questions about the enzymatic regulation of this widespread post-translational modification. In this review we will discuss the impact of new chemical labeling approaches and future challenges for the dynamic global analysis of protein palmitoylation.