The design and characterization of a gravitational microfluidic platform for drug sensitivity assay in colorectal perfused Tumoroid cultures.
The design and characterization of a gravitational microfluidic platform for drug sensitivity assay in colorectal perfused Tumoroid cultures.
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DOI：
10.1016/j.nano.2020.102294
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发表时间：
2020-08
期刊：
影响因子：
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通讯作者：
Tao Wang;R. Green;M. Howell;T. Martinez;Rinku Dutta;S. Mohapatra;S. Mohapatra
中科院分区：
文献类型：
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作者：
Tao Wang;R. Green;M. Howell;T. Martinez;Rinku Dutta;S. Mohapatra;S. Mohapatra
肿瘤微环境在肿瘤的发生、发展、转移和耐药性中起着关键作用。然而，模型重现复杂的3D结构，异质性细胞环境，和细胞间相互作用在体内发现缺乏。在这里，我们报告了一个重力微流控平台（GMP）改造与MEMS传感器，这是集成了三维支架辅助类肿瘤培养。结果表明，这种用于类肿瘤生长的GMP比结肠癌的静态培养模型更精确地模拟肿瘤微环境，包括更高的耐药性、增强的癌症干细胞特性和增加的促肿瘤细胞因子分泌。此外，GMP还包括一个集成的基于表面声波的生物传感器，用于监测细胞生长和pH值变化，以评估药物疗效。因此，这种用于体外和体内研究的简单易用的灌注GMP类肿瘤培养系统可能会加速药物开发过程，并成为个性化癌症治疗的工具。
The tumor microenvironment plays a critical role in tumor initiation, progression, metastasis, and drug resistance. However, models recapitulating the complex 3D structure, heterogeneous cell environment, and cell-cell interactions foundin vivoare lacking. Herein, we report on a gravitational microfluidic platform (GMP) retrofitted with MEMS sensors, which is integrated with 3D nanofiber scaffold-aided tumoroid culture. The results showed that this GMP for tumoroid growth mimics the tumor microenvironment more precisely than static culture models of colon cancer, including higher drug resistance, enhanced cancer stem cell properties, and increased secretion of pro-tumor cytokines. In addition, the GMP includes an integrated surface acoustic wave-based biosensing to monitor cell growth and pH changes to assess drug efficacy. Thus, this simple-to-use perfused GMP tumoroid culture system forin vitroandex vivostudies may accelerate the drug development process and be a tool in personalized cancer treatment.