Detection of carbohydrates by electrospray ionization ion mobility spectrometry following microbore high-performance liquid chromatography
Detection of carbohydrates by electrospray ionization ion mobility spectrometry following microbore high-performance liquid chromatography
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DOI：
10.1016/s0021-9673(98)00486-5
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发表时间：
1998-09-25
影响因子：
4.1
通讯作者：
Hill, HH
中科院分区：
文献类型：
--
作者：
Lee, DS;Wu, C;Hill, HH
本文报道了电喷雾离子迁移谱作为 HPLC 分离检测方法的第一个实例，展示了其在定量和选择性检测非挥发性和非发色有机化合物方面的潜力。 21 种碳水化合物（包括单糖、糖醇和氨基糖）的折合迁移率常数 (K-o) 经测定范围为 0.68 至 1.37 cm(2) V-1 s(-1)。测得的最低可检测量从低至 5.8.10(-14) mol（D(+)-纤维二糖）到高达 8.2.10(-11) mol（L-艾杜醇）。碳水化合物质量相对于反离子迁移率的图表明，对于大多数碳水化合物来说，对于第一近似值，迁移率与质量成反比。然而，在某些情况下，异构体分离可以通过离子淌度光谱法实现，这表明离子淌度分离是基于分子的大小和形状而不是分子质量。迁移率与质量关系的其他值得注意的例外是 β-D-麦芽糖和 D(+)-纤维二糖，其迁移率明显低于预期，而麦芽七糖则表现出明显高于预期的迁移率。二聚化和多重电荷可能导致这些与简单的质量迁移率相关性的偏差。参数优化加上对这种电离和检测方法的进一步基础研究，应该会产生一种分析测定碳水化合物的新方法。 (C) 1998 Elsevier Science B.V. 保留所有权利。
This paper reports the first example of electrospray ion mobility spectrometry as a detection method for HPLC separation, demonstrating its potential for the quantitative and selective detection of non-volatile and non-chromophoric organic compounds. Reduced mobility constants (K-o) for 21 carbohydrates, including simple sugars, sugar alcohols and amino sugars, were determined to range from 0.68 to 1.37 cm(2) V-1 s(-1). Minimum detectable quantities were measured from as low as 5.8.10(-14) mol for D(+)-cellobiose to as high as 8.2.10(-11) mol for L-iditol. A plot of carbohydrate mass with respect to inverse ion mobility indicated that, to a first approximation, mobility was inversely proportional to mass for most carbohydrates. However, in several cases, isomeric separation could be achieved by ion mobility spectrometry, demonstrating that ion mobility separation is based on size and shape of the molecule rather than molecular mass. Other notable exceptions to the mobility-mass relation were beta-D-maltose and D(+)-cellobiose, which exhibited significantly lower mobilities than expected, and maltoheptaose, which exhibited a significantly higher mobility than expected. Dimerization and multiple charges may have caused these deviations from the simple mass-mobility correlation. Parametric optimization coupled with further basic investigations of this ionization and detection method should lead to a novel approach for the analytical determination of carbohydrates. (C) 1998 Elsevier Science B.V. All rights reserved.