TopoStats - a program for automated tracing of biomolecules from AFM images
TopoStats - a program for automated tracing of biomolecules from AFM images
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TopoStats - 从 AFM 图像自动追踪生物分子的程序
DOI：
10.1101/2020.09.23.309609
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Beton J
中科院分区：
文献类型：
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作者：
Beton J
TopoStats是一个Python工具包，用于自动编辑和分析原子力显微镜图像。该程序包括识别和跟踪单个分子的环状和线性构象，无需用户输入。该程序可通过GitHub（https：//github.com/afmstats/TopoStats）免费获得，并可根据需要进行修改和调整。TopoStats可以在宽视场内识别单个分子和分子组装体，而无需事先处理。我们通过识别和追踪单个生物分子，包括DNA折纸，孔形成蛋白质和闭合环状和线性形式的DNA分子来展示其功能。
We present TopoStats, a Python toolkit for automated editing and analysis of Atomic Force Microscopy images. The program includes identification and tracing of individual molecules in both circular and linear conformations without user input. The program is freely available via GitHub (https://github.com/afmstats/TopoStats), and is intended to be modified and adapted for use if required. TopoStats can identify individual molecules and molecular assemblies within a wide field of view, without the need for prior processing. We demonstrate its power by identifying and tracing individual biomolecules, including DNA origami, pore-forming proteins, and DNA molecules in both closed circular and linear form.