Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae
Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae
复制标题
麻风寄生虫麻风分枝杆菌主要蛋白抗原的基因
作者：
R. Young;V. Mehra;D. Sweetser;T. Buchanan;J. Clark;Ronald W. Davis;B. Bloom
麻风病是一种慢性传染病，影响着1000万至1500万人，由专性细胞内寄生虫麻风分枝杆菌引起。虽然麻风分枝杆菌是第一个被发现的人类细菌病原体，但由于它仍然是少数未在体外培养的人类病原体之一，因此基本的生化、免疫学、诊断和治疗研究受到严重限制。构建麻风分枝杆菌重组DNA表达文库，为此类研究提供相关蛋白编码基因来源。针对麻风杆菌特异性抗原s3 - 7的单克隆抗体已被用于分离麻风杆菌5种最具免疫原性的蛋白抗原的编码基因。我们在此报告，所有13个单克隆抗体所识别的麻风分枝杆菌特异性表位是由重组噬菌体在大肠杆菌中产生的。
Leprosy, a chronic infectious disease afflicting between 10 and 15 million people, is caused by the obligate intracellular parasite Mycobacterium leprae1. Although M. leprae was the first identified bacterial pathogen of man2, basic biochemical, immunological, diagnostic and therapeutic investigations have been severely limited because it remains one of the few human pathogens that have not been cultured in vitro. An M. leprae recombinant DNA expression library was constructed to provide a source of genes encoding proteins relevant for such studies. Monoclonal antibodies directed against M. leprae specific antigens3–7 have been used to isolate the genes encoding the five most immunogenic protein antigens of the leprosy bacillus. We report here that M. leprae specific epitopes recognized by all of 13 monoclonal antibodies tested were produced by recombinant phage in Escherichia coli.
使用重组 DNA 解剖结核分枝杆菌抗原。
DOI：
10.1073/pnas.82.9.2583
发表时间：
1985
影响因子：
11.1
作者：
Young,RA;Bloom,BR;Grosskinsky,CM;Ivanyi,J;Thomas,D;Davis,RW
通讯作者：
Davis,RW
DOI：
10.1073/pnas.80.5.1194
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
YOUNG, RA;DAVIS, RW
通讯作者：
DAVIS, RW