Translocation of bacteria from the gastrointestinal tract: protection afforded by lisofylline.
Translocation of bacteria from the gastrointestinal tract: protection afforded by lisofylline.
复制标题
细菌从胃肠道的移位：利索茶碱提供的保护。
DOI：
10.1016/s0041-1345(98)01561-9
复制
发表时间：
1999
影响因子：
0.9
通讯作者：
Starzl,TE
中科院分区：
文献类型：
--
作者：
Cicalese,L;Yacoub,W;Rogers,J;Fung,JJ;Rao,AS;Starzl,TE
材料和方法本研究使用体重为200 - 250 g的雄性ACI大鼠（哈兰Sprague道利）。通过无菌夹闭上级肠系膜动脉30和45分钟使小肠（SB）缺血，同时使动物保持常温（37 ℃）。对SB进行再灌注，并缝合腹部。手术后24小时采集血液、肠系膜淋巴结（MLN）、脾、肝、肺和胰腺标本进行定量和定性微生物分析。在剖腹手术时（对照）、手术结束时腹部闭合前（污染对照）和处死时进行腹膜拭子培养。在损伤之前和之后，在研究组中静脉内（IV）施用LSF（25 mg/kg），而对照动物接受等体积的无菌盐水。在饮用水实验前24小时给予大鼠荧光乳胶微球的易位，用于确认易位颗粒的肠来源，并通过腹膜灌洗液上的荧光激活细胞分选仪进行评估。还通过组织病理学分析评估SB粘膜变化。使用假手术未处理的动物作为对照（n= 6）。在无阻尼动物（n= 4）上测试单独的LSF的作用，而在未处理的大鼠（分别为n= 6; n= 10）和LSF处理的大鼠（分别为n = 6; n= 9）之间评价肠缺血30和45分钟对BT的作用。Fisher精确检验用于比较阳性培养物的比例，P值小于。结果表1中总结的数据表示为器官培养大肠埃希氏菌和/或肠球菌（> 1× 104个细菌）或腹膜拭子菌落超过300个的动物百分比（P<. 05）。
MATERIALS AND METHODSMale ACI rats weighing 200 to 250 g (Harlan Sprague Dawley) were used for the study. The small bowel (SB) was rendered ischemic by aseptically clamping the superior mesenteric artery for 30 and 45 minutes while the animals were kept euthermic (37 C). The SB was reperfused and the abdomen was sutured. Blood, mesenteric lymph nodes (MLN), spleen, liver, lung, and pancreas specimens were collected 24 hours after surgery for quantitative and qualitative microbiologic analysis. Peritoneal swab cultures were performed at the time of laparotomy (control), at the end of surgery before abdominal closure (contamination control), and at sacrifice. LSF (25 mg/kg) was administered intravenously (IV) in the study groups before and after the insult while control animals received equal volume of sterile saline. Translocation of fluorescent latex microspheres, administered to the rats 24 hours before experimentation in drinking water, was used to confirm the intestinal origin of translocating particles and was assessed by fluorescent-activated cell sorter on peritoneal lavages. SB mucosal changes were also assessed by histopathological analysis. Sham untreated animals were used as controls (n= 6). The effect of LSF alone was tested on undamped animals (n= 4) while the effect of 30 and 45 minutes of intestinal ischemia on BT was evaluated between untreated (n= 6; n= 10, respectively) and LSF-treated rats (n= 6; n= 9, respectively). Fisher Exact Test was used to compare the proportions of positive cultures and a P value of less than. 05 was considered statistically significant.RESULTSThe data summarized in Table 1 is expressed as a percentage of animals with positive organ cultures for Erischeria-coli and/or enterococcus (> 1× 10 4 bacteria) or peritoneal swabs with more than 300 colonies (P<. 05).