Occlusal hypofunction causes changes of proteoglycan content in the rat periodontal ligament
Occlusal hypofunction causes changes of proteoglycan content in the rat periodontal ligament
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DOI：
10.1034/j.1600-0765.2001.00607.x
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发表时间：
2001-02-01
影响因子：
3.5
通讯作者：
Yanagishita, M
中科院分区：
文献类型：
--
作者：
Kaneko, S;Ohashi, K;Yanagishita, M
蛋白聚糖和糖胺聚糖的生物学功能与组织上的机械应力密切相关。为了揭示牙周膜中蛋白聚糖与咬合刺激等机械应力之间的关系，通过拔除对侧上颌第一、第二和第三磨牙来诱导大鼠单侧下颌磨牙咬合功能低下。使用硫酸软骨素、核心蛋白聚糖、双糖链蛋白聚糖、硫酸乙酰肝素和硫酸角质素以及透明质酸结合蛋白的抗体进行免疫组织化学分析。硫酸软骨素，观察到更强烈地在颈部侧比在根尖侧的牙周膜的未提取侧的下颌骨，并均匀地存在于牙周膜的细胞外基质中，从1周后提取的拮抗剂显着下降，在纤维成分的厚度和紊乱的减少。核心蛋白聚糖核心蛋白，均匀地存在于牙周膜的未提取侧，减少早在2天后提取。硫酸乙酰肝素主要定位于血管内皮细胞和骨细胞的细胞表面以及未拔牙侧的细胞外基质中，早在拔牙后2 d就与血管和骨细胞数量的减少显著相关。双糖链、硫酸角质素和透明质酸在未拔牙侧牙周膜中分布均匀，拔牙后变化不大。这些：结果表明，咬合功能减退导致牙周膜组织重塑，硫酸软骨素、核心蛋白聚糖和硫酸乙酰肝素显著减少。
The biological functions of proteoglycans and glycosaminoglycans are closely associated with mechanical stress on the tissue. In order to reveal the relationship between proteoglycans in the periodontal ligament and mechanical stress such as occlusal stimuli, occlusal hypofunction of rat unilateral mandibular molars was induced by extraction of the opposing first, second and third maxillary molars. Immunohistochemical analyses were performed using antibodies for chondroitin sulfate, decorin, biglycan, heparan sulfate and keratan sulfate, and hyaluronic acid-binding protein. Chondroitin sulfate, observed more strongly in the cervical side than in the apical side of the periodontal ligament of the unextracted sides of mandible, and uniformly present in the extracellular matrix of the periodontal ligament, decreased significantly from I wk post-extraction of the antagonists, with a decrease in thickness and disarrangement in fibrous components. Decorin core protein, uniformly present in the periodontal ligament of the unextracted sides, decreased as early on as 2 d post-extraction. Heparan sulfate, mainly localized on the cell surface of vascular endothelial cells and osteoclastic cells as well as in the extracellular matrix of the unextracted sides, decreased significantly in association with the decreased number of blood vessels and osteoclastic cells as early on as 2 d post-extraction. Biglycan, keratan sulfate and hyaluronic acid, uniformly distributed in the periodontal ligament of the unextracted sides, showed little change after the extraction. These: results demonstrate that occlusal hypofunction causes tissue remodeling of the periodontal ligament, with a significant decrease of chondroitin sulfate, decorin and heparan sulfate.