Inverted light-sheet microscope for imaging mouse pre-implantation development
Inverted light-sheet microscope for imaging mouse pre-implantation development
复制标题
DOI：
10.1038/nmeth.3690
复制
发表时间：
2016-02-01
期刊：
影响因子：
48
通讯作者：
Ellenberg, Jan
中科院分区：
文献类型：
--
作者：
Strnad, Petr;Gunther, Stefan;Ellenberg, Jan
尽管它的重要性，了解人类不育症和先天性疾病，早期哺乳动物的发展仍然无法在全成像。我们开发了一种倒置光片显微镜，使我们能够从受精卵到囊胚对小鼠胚胎进行成像，计算跟踪所有细胞并重建小鼠植入前发育的完整谱系树。我们使用这一独特的数据集表明，第一个细胞命运的规范发生在16细胞阶段。
Despite its importance for understanding human infertility and congenital diseases, early mammalian development has remained inaccessible to in toto imaging. We developed an inverted light-sheet microscope that enabled us to image mouse embryos from zygote to blastocyst, computationally track all cells and reconstruct a complete lineage tree of mouse pre-implantation development. We used this unique data set to show that the first cell fate specification occurs at the 16-cell stage.