PROTEIN SPLICING CONVERTS THE YEAST TFP1 GENE-PRODUCT TO THE 69-KD SUBUNIT OF THE VACUOLAR H+-ADENOSINE TRIPHOSPHATASE
PROTEIN SPLICING CONVERTS THE YEAST TFP1 GENE-PRODUCT TO THE 69-KD SUBUNIT OF THE VACUOLAR H+-ADENOSINE TRIPHOSPHATASE
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DOI：
10.1126/science.2146742
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发表时间：
1990-11-02
期刊：
影响因子：
56.9
通讯作者：
STEVENS, TH
中科院分区：
文献类型：
--
作者：
KANE, PM;YAMASHIRO, CT;STEVENS, TH
酿酒酵母的TFP 1基因编码两种蛋白：液泡质子转运腺苷三磷酸酶（H+-ATPase）的69 kD催化亚基和50 kD蛋白。69-kD亚基由TFP 1编码区的5“和3”三分之一编码，而50-kD蛋白由中央三分之一编码。有证据表明，69 kD和50 kD的蛋白质是从一个单一的翻译产物，被切割释放的50 kD的蛋白质和剪接，形成69 kD的亚基。
The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H+-ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5'' and 3'' thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit.