Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen, Rhizoctonia solani
Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen, Rhizoctonia solani
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DOI：
10.1007/s10529-012-1035-z
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发表时间：
2012-12-01
影响因子：
2.7
通讯作者：
Sun, Guochang
中科院分区：
文献类型：
--
作者：
Liu, He;Tian, Wenxiao;Sun, Guochang
研究了3种壳聚糖对水稻纹枯病病原菌茄枯丝核菌的抑菌性能及抑菌机理。两种壳聚糖均具有较强的抗真菌活性，对水稻纹枯病有一定的保护作用，其中两种酸溶性壳聚糖对菌丝生长的抑制作用为60- 91%，对病害发生率的抑制作用为31- 84%，对病害长度的抑制作用为66- 91%。扫描电镜观察、透射电镜观察和致病性试验结果表明，壳聚糖对水稻病原菌具有直接破坏作用；水稻幼苗防御相关的苯丙氨酸解氨酶、过氧化物酶和多酚氧化酶活性的变化证明了间接诱导抗性。据我们所知，这是首次报道壳聚糖对稻瘟病菌的抗真菌活性。
The antifungal properties and mechanism of three types of chitosan against the rice sheath blight pathogen, Rhizoctonia solani, were evaluated. Each chitosan had strong antifungal activity against R. solani and protected rice seedlings from sheath blight, in particular, two types of acid-soluble chitosan caused a 60-91 % inhibition in mycelial growth, 31-84 % inhibition of disease incidence, and 66-91 % inhibition in lesion length. The mechanism of chitosan in protection of rice from R. solani pathogen was attributed to direct destruction of the mycelium, evidenced by scanning and transmission electron microscopic observations and pathogenicity testing; indirect induced resistance was evidenced by the changes in the activities of the defense-related phenylalanine ammonia lyase, peroxidase and polyphenol oxidase in rice seedling. To our knowledge, this is the first report on the antifungal activity of chitosan against rice R. solani.