Immunohistochemical localization of enkephalin in rat brain and spinal cord
Immunohistochemical localization of enkephalin in rat brain and spinal cord
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大鼠脑和脊髓中脑啡肽的免疫组织化学定位
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
P. Cuatrecasas
中科院分区：
文献类型：
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作者：
M. Sar;Walter E. Stumpf;Richard J. Miller;Kwen;P. Cuatrecasas
采用对亮氨酸-脑啡肽（[Leu5] -脑啡肽）或蛋氨酸-脑啡肽（[Met5] -脑啡肽）的抗血清免疫过氧化物酶染色，研究了免疫反应性脑啡肽在大鼠脑和脊髓中的分布。在中枢神经系统许多区域的神经纤维、末梢和细胞体中，两种脑啡肽的免疫反应性染色相似。去垂体大鼠和秋水仙碱预处理大鼠核周染色。脑啡肽纤维和脑末梢的定位区域包括：前脑外侧隔、杏仁核中央核、海马CA2区、皮层的某些区域、纹状体、终纹床核、下丘脑（包括正中隆起）、丘脑和丘脑下；中脑：核柄间核、导水管周围灰质和网状结构；后脑：臂旁核、蓝核、脑核、耳蜗核、孤束核、三叉神经脊髓核、部分脑神经运动核、联合核、网状结构核；脊髓中有胶状物质。而脑啡肽细胞体则稀疏分布于远脑、间脑、前脑和右脑。组织化学染色结果显示，脑啡肽阳性染色的某些结构与阿片受体在大脑中的分布密切相关，从而支持内源性阿片肽参与疼痛和镇痛感知的概念。脑啡肽在视前-下丘脑区域的定位以及脑啡肽在核周室旁核和视上核的存在表明脑啡肽在调节神经内分泌功能中的作用。
The distribution of immunoreactive enkephalin in rat brain and spinal cord was studied by immunoperoxidase staining using antiserum to leucine‐enkephalin ([Leu5]‐enkephalin) or methionine‐enkephalin ([Met5]‐enkephalin). Immunoreactive staining for both enkephalins was similarly observed in nerve fibers, terminals and cell bodies in many regions of the central nervous system. Staining of perikarya was detected in hypophysectomized rats or colchicine pretretated rats. The regions of localization for enkephalin fibers and terminals include in the forebrain: lateral septum, central nucleus of the amygdala, area CA2 of the hippocampus, certain regions of the cortex, corpus striatum, bed nucleus of the stria terminalis, hypothalamus including median eminence, thalamus and subthalamus; in the midbrain: nucleus interpeduncularis, periaqueductal gray and reticular formation; in the hind brain: nucleus parabrachialis, locus ceruleus, nuclei raphes, nucleus cochlearis, nuclear tractus solitarii, nucleus spinalis nervi trigemini, motor nuclei of certain cranial nerves, nucleus commissuralis and formatio reticularis; and in the spinal cord the substantia gelatinosa. In contrast enkephalin cell bodies appear sparsely distributed in the telencephalon, diencephelon, mensencephalon and rhombencephalon. The results of the histochemical staining show that certain structures which positively stain for enkephalin closely correspond to the distribution of opiate receptors in the brain and thus support the concept that the endogenous opiate peptides are involved in the perception of pain and analgesia. The localization of enkephalin in the preoptic‐hypothalamic region together with the presence of enkephalin perikarya in the paraventricular and supraoptic nuclei suggest a role of enkephalin in the regulation of neuroendocrine functions.