CHARACTERIZATION OF A DETERGENT-RESISTANT SURFACE LAMINA IN CULTURED HUMAN-FIBROBLASTS
CHARACTERIZATION OF A DETERGENT-RESISTANT SURFACE LAMINA IN CULTURED HUMAN-FIBROBLASTS
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DOI：
10.1016/0014-4827(83)90053-8
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发表时间：
1983-01-01
影响因子：
3.7
通讯作者：
VIRTANEN, I
中科院分区：
文献类型：
--
作者：
LEHTO, VP;VARTIO, T;VIRTANEN, I
用 0.5% Triton X-100 处理培养的人成纤维细胞，产生由细胞质丝、细胞核和质膜衍生的表面层组成的基质锚定细胞骨架制剂。在荧光显微镜下，用荧光染料偶联的小麦胚芽凝集素（WGA）将层层可视化为花边状结构，延伸到整个细胞域。它在细胞的腹侧和背侧表面显示出不同的组织，与背侧细胞表面的肌动蛋白和肌球蛋白丝束部分对齐。在腹侧表面，纽蛋白斑块似乎包含在表面层中。聚丙烯酰胺凝胶电泳结合凝集素反应性研究和凝集素亲和层析，揭示了 140 kD [千道尔顿]唾液酸糖蛋白是表面层的主要糖蛋白成分。
Treatment of cultured human fibroblasts with 0.5% Triton X-100 produces substratum-anchored cytoskeletal preparations consisting of cytoplasmic filaments, nucleus and a plasma membrane-derived surface lamina. The lamina was visualized in fluorescence microscopy with fluorochrome-coupled wheat germ agglutinin (WGA) as a lace-like structure, extending throughout the cell domain. It displayed a different organization at the ventral and dorsal surfaces of the cell, partially coaligning with bundles of actin and myosin filaments at the dorsal cell surface. At the ventral surface vinculin patches appeared to be included in the surface lamina. Polyacrylamide gel electrophoresis, combined with lectin reactivity studies and lectin affinity chromatography, revealed a 140 kD [kilodalton] sialoglycoprotein as the major glycoprotein component of the surface lamina.