Antibacterial activity of gold-titanates on Gram-positive cariogenic bacteria.
Antibacterial activity of gold-titanates on Gram-positive cariogenic bacteria.
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DOI：
10.3109/23337931.2015.1084883
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发表时间：
2015-12-01
影响因子：
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通讯作者：
Chan, Daniel C N
中科院分区：
文献类型：
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作者：
Eiampongpaiboon, Trinuch;Chung, Whasun O;Chan, Daniel C N
目的：革兰氏阳性致龋细菌是龋齿的病原体;因此，抑制这些细菌以减少这种疾病的发生的策略已经加强。本文研究了钛酸盐和金钛酸盐对干酪乳杆菌（Lactobacillus casei，Lc）和变形链球菌（Streptococcus mutans，Sm）的抗菌活性。材料与方法：钛酸一钠（MST）、纳米钛酸一钠（nMST）和无定形过氧钛酸盐（APT）是对特定金属离子具有高结合亲和力的无机化合物。测量总细菌蛋白以代表与金钛酸盐孵育24小时后的细菌细胞质量。我们通过活/死荧光染色和集落形成单位（CFU）进一步检查了nMST-Au（III）浓度（10、200、400 mg/L）随时间对Lc和Sm细胞活力的影响。透射电子显微镜（TEM）用于确定受nMST-Au（III）影响的细菌细胞上的特定位置。结果：我们发现所有的金钛酸盐和单独的APT都降低了细菌蛋白的Lc（p值
Objective: Gram-positive cariogenic bacteria are etiological agents in dental caries; therefore, strategies to inhibit these bacteria to reduce the incident of this disease have intensified. In this study, we investigated antibacterial activities of titanates and gold-titanates against Lactobacillus casei (Lc) and Streptococcus mutans (Sm). Materials and methods: Monosodium titanate (MST), nanomonosodium titanate (nMST) and amorphous peroxo-titanate (APT), which are inorganic compounds with high-binding affinity for specific metal ions, were used. Total bacterial proteins were measured to represent bacterial cell mass after 24 h incubation with gold-titanates. We further examined the effect of nMST-Au(III) concentrations (10,200,400 mg/L) on Lc and Sm cell viability over time via Live/Dead fluorescent staining and colony forming units (CFUs). Transmission electron microscopy (TEM) was used to determine specific locations on the bacterial cells affected by the nMST-Au(III). Results: We found all gold-titanates and APT alone reduced bacterial protein for Lc (p value