Estimation of Molecular Hydrogen Consumption in the Human Whole Body After the Ingestion of Hydrogen-Rich Water
Estimation of Molecular Hydrogen Consumption in the Human Whole Body After the Ingestion of Hydrogen-Rich Water
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DOI：
10.1007/978-1-4614-1566-4_36
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Kondo, Takaharu
中科院分区：
文献类型：
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作者：
Shimouchi, Akito;Nose, Kazutoshi;Kondo, Takaharu
最近的研究表明，在动物模型中，吸入或摄入氢气（H2）可使各种疾病和病症中的活性氧（如羟基自由基）失活，并且H2可减少脑、心脏和其他外周组织中氧化应激诱导的损伤。这些报道表明外源H2部分被氧自由基捕获。本研究旨在评估摄入富含H2的水后的H2消耗量。7名成年受试者摄入富含H2的水。用半导体气相色谱法测定呼气中的H2含量。摄入富含H2的水迅速增加呼吸H2含量约36 ppm的最大水平，在摄入后10分钟，此后减少到基线水平在60分钟内。同时测量呼气分钟量一起，59%的摄入H2呼出。在实验过程中从水中损失的H2占H2的3%或更少。从皮肤表面释放的H2估计为约0.1%。基于剩余的H2质量平衡，大约40%的摄入的H2在体内消耗。据报告，H2分子是羟基自由基的弱清除剂，对超氧化物或过氧化氢无效，因此，假设H2分子全部用于清除羟基自由基，并且可以排除消化道和皮肤表面的细菌消耗，则羟基自由基产生速率估计至少为1.0 μmol/min/m2（相当于29 nmol/min/kg）。总之，摄入的H2有59%被呼出，其余大部分在体内消耗。
Recent studies have revealed that inhaled or ingested hydrogen gas (H2) inactivates reactive oxygen species such as hydroxyl radicals in various kinds of diseases and disorders in animal models and that H2reduces oxidative stress-induced damage in brain, heart, and other peripheral tissues. These reports suggested that exogenous H2is partially trapped by oxygen radicals. This study was conducted to evaluate H2consumption after the ingestion of H2-rich water. Seven adult subjects ingested H2-rich water. The H2content of their expired breath was measured by gas chromatography with a semiconductor. The ingestion of H2-rich water rapidly increased breath H2content to its maximal level of approximately 36 ppm at 10 min after ingestion and thereafter decreased it to the baseline level within 60 min. Taken together with simultaneous measurements of expiratory minute volume, 59% of the ingested H2was exhaled. The loss of H2from the water during the experimental procedures accounted for 3% or less of the H2. H2release from the skin surface was estimated as approximately 0.1%. Based on the remaining H2mass balance, approximately 40% of the ingested H2was consumed in the body. As the H2molecule is reported to be a weak scavenger of hydroxyl radicals and is not effective against superoxide or hydrogen peroxide, the rate of hydroxyl radical production was estimated to be at least 1.0 μmol/min/m2(equivalent to 29 nmol/min/kg), assuming that the H2molecules were all used to scavenge hydroxyl radicals and that bacterial consumption in the alimentary tract and on the skin surface could be excluded. In summary, 59% of ingested H2was exhaled, and most of the remainder was consumed in the body.