RELATION OF COUNTS OF MICROBIAL SPECIES TO CLINICAL STATUS AT THE SAMPLED SITE
RELATION OF COUNTS OF MICROBIAL SPECIES TO CLINICAL STATUS AT THE SAMPLED SITE
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DOI：
10.1111/j.1600-051x.1991.tb00070.x
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发表时间：
1991-11-01
影响因子：
6.7
通讯作者：
DIBART, S
中科院分区：
文献类型：
--
作者：
SOCRANSKY, SS;HAFFAJEE, AD;DIBART, S
本调查的目的是在一个网站的检测频率，绝对计数和比例的14个龈下物种的临床特征。用刮匙从3名健康受试者和87名牙周附着丧失受试者的2299颗牙齿的近中面去除龈下菌斑样本。将样品分散、稀释并铺板在补充有5%羊血的胰蛋白胨大豆琼脂上。厌氧培养7天后，将菌落提升到尼龙滤器上，裂解并将DNA固定到滤器上。地高辛标记的DNA探针用于鉴定每个试验种属的菌落。在每个取样部位测量囊袋深度、附着水平、退缩、发红、探诊出血和化脓。研究中心的总活菌计数范围为10（3）至> 10（8），与囊袋深度密切相关。< 3 mm部位的平均总计数为4.6 x 10（6），而> 7 mm部位的平均计数为2.0 x 10（7）。所列举的物种和定殖位点的百分比如下：小弧菌44; sanguis II 36; B. intermedius I 33; C.赭色31; B. intermedius II 30; S. sanguis I 29; B. gingivalis 27; S. intermedius 25; P.micros24; W. recta 23; F. nucleatum ss vincentii 18; B. 15; A.伴放线菌血清型a10;伴放线菌血清型B 8.数B。中间肌II在牙龈发红的部位更高，而B。intermedius I在探测流血的的部位更高。A.伴放线菌血清型B更常见，在没有衰退的地点平均百分比更高。S的情况正好相反。血二B。牙龈炎较为普遍，化脓部位的牙龈炎水平较高。B。gingivalis，B. B.中间体I和II和B。在囊袋较深的部位，更频繁地发现小弧菌，且水平较高，而在囊袋深度< 4 mm的部位，小弧菌不太常见。B。gingivalis，B. B. intermedius I和A.伴放线菌血清型B在局限性和广泛性疾病受试者中均随囊袋深度的增加而增加，但在任何囊袋深度的局限性疾病受试者中平均计数均较高。只有W。与局限性疾病受试者的类似部位相比，在广泛性疾病受试者的深部部位发现直肠癌的水平较高。在38%的浅部位、31%的中间部位和22%的深部位中未检测到可疑病原体。2/3的深袋，但不到1/2的浅袋窝藏至少2种可疑病原体。
The purpose of the present investigation was to relate clinical characteristics at a site to the frequency of detection, absolute counts and proportions of 14 subgingival species. Subgingival plaque samples were removed by curette from the mesial surface of 2299 teeth in 3 healthy and 87 subjects with periodontal attachment loss. Samples were dispersed,diluted and plated on Trypticase soy agar supplemented with 5% sheep blood. After 7 days of anaerobic incubation, colonies were lifted onto nylon filters, lysed and the DNA fixed to the filters. Digoxygenin-labeled DNA probes were used to identify colonies of each test species. Measurements of pocket depth, attachment level, recession, redness, bleeding on probing and suppuration were made at each sampled site. Total viable counts at sites ranged from 10(3) to > 10(8) and were strongly related to pocket depth. Mean total counts at sites < 3 mm averaged 4.6 x 10(6), while mean counts at sites > 7 mm averaged 2.0 x 10(7). Species enumerated and % of sites colonized were as follows; V. parvula 44; S. sanguis II 36; B. intermedius I 33; C. ochracea 31; B. intermedius II 30; S. sanguis I 29; B. gingivalis 27; S. intermedius 25; P. micros 24; W. recta 23; F. nucleatum ss vincentii 18; B. forsythus 15; A. actinomycetemcomitans serotype a 10; A. actinomycetemcomitans serotype b 8. Counts of B. intermedius II were higher at sites which exhibited gingival redness while B. intermedius I was higher at sites which bled on probing. A. actinomycetemcomitans serotype b was more frequent and at higher mean % at sites without recession. The opposite was true for S. sanguis II. B. gingivalis was somewhat more prevalent and at higher levels at suppurating sites. B. gingivalis, B. intermedius I and II and B. forsythus were found more frequently and at higher levels at sites with deeper pockets, while, V. parvula was less prevalent at sites with pocket depths < 4 mm. B. gingivalis, B. intermedius I and A. actinomycetemcomitans serotype b increased with increasing pocket depth in both localized and widespread disease subjects, but mean counts were higher in the localized disease subjects at any pocket depth. Only W. recta was found at higher levels at deep sites in widespread disease subjects when compared with similar sites in localized disease subjects. No suspected pathogens were detected in 38% of shallow sites, 31% of intermediate sites and 22% of deep sites. 2/3 of deep pockets, but less than 1/2 of shallow pockets harbored at least 2 of the suspected pathogens.