In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria.
In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria.
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DOI：
10.1590/1678-775720160044
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Cogo-Müller K
中科院分区：
文献类型：
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作者：
Graziano TS;Calil CM;Sartoratto A;Franco GC;Groppo FC;Cogo-Müller K
口臭可由产生挥发性硫化合物（VSC）的微生物引起，这些挥发性硫化合物定植在舌头表面和龈下部位。研究报告说，使用天然产品可以减少细菌负荷，从而减少口臭的发展。本研究的目的是评价互叶白千层精油对口腔细菌生长和挥发性硫化物（VSC）产生的抗菌活性与洗必泰相比。 以牙龈卟啉单胞菌和牙髓卟啉单胞菌体外培养的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）评价这些物质的作用。此外，进行气相色谱分析以测量来自细菌培养物的VSCs的浓度并表征M.互隔叶油成分 MIC和MBC值如下：M.互叶假单胞菌-牙龈卟啉单胞菌（MIC和MBC=0.007%）、牙髓卟啉单胞菌（MIC和MBC=0.007%=0.5%）;洗必泰-牙龈卟啉单胞菌和牙髓卟啉单胞菌（MIC和MBC=1.5 mg/mL）。M. alternifolia显著降低牙龈卟啉单胞菌的生长和硫化氢（H2S）产生（p<0.05，ANOVA-Dunnet）以及牙髓卟啉单胞菌的H2S和甲基硫（CH 3SH）水平（p<0.05，ANOVA-Dunnet）。洗必泰减少了两种微生物的生长，而不改变牙髓普氏菌中VSC的产生。对于牙龈卟啉单胞菌，H2S和CH 3SH的产生减少（p<0.05，AN 0 VA-Dunnet）。 M. alternifolia可以减少细菌生长和VSCs产生，并且可以用作氯己定的替代品。
Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of halitosis. The aim of this study was to evaluate the antimicrobial activity of the essential oil of Melaleuca alternifolia on the growth and volatile sulphur compound (VSC) production of oral bacteria compared with chlorhexidine. The effects of these substances were evaluated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in planktonic cultures of Porphyromonas gingivalis and Porphyromonas endodontalis. In addition, gas chromatography analyses were performed to measure the concentration of VSCs from bacterial cultures and to characterize M. alternifolia oil components. The MIC and MBC values were as follows: M. alternifolia - P. gingivalis (MIC and MBC=0.007%), P. endodontalis (MIC and MBC=0.007%=0.5%); chlorhexidine - P. gingivalis and P. endodontalis (MIC and MBC=1.5 mg/mL). M. alternifolia significantly reduced the growth and production of hydrogen sulfide (H2S) by P. gingivalis (p<0.05, ANOVA-Dunnet) and the H2S and methyl mercaptan (CH3SH) levels of P. endodontalis (p<0.05, ANOVA-Dunnet). Chlorhexidine reduced the growth of both microorganisms without altering the production of VSC in P. endodontalis. For P. gingivalis, the production of H2S and CH3SH decreased (p<0.05, ANOVA-Dunnet). M. alternifolia can reduce bacterial growth and VSCs production and could be used as an alternative to chlorhexidine.