The interactive effect of water deficit and UV-B radiation on salicylic acid accumulation in barley roots and leaves
The interactive effect of water deficit and UV-B radiation on salicylic acid accumulation in barley roots and leaves
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DOI：
10.1016/j.envexpbot.2012.03.001
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发表时间：
2013-10-01
影响因子：
5.7
通讯作者：
Cieslak, Malgorzata
中科院分区：
文献类型：
--
作者：
Bandurska, Hanna;Cieslak, Malgorzata
研究了水分亏缺（WD）和UV-B辐射单独和联合作用对水杨酸（SA）积累以及苯丙氨酸解氨酶（PAL）和苯甲酸羟化酶（BA 2 H）活性的影响。另一个目的是测试这些应力的相互作用是否限制了单一应力对组织水合作用和膜损伤的负面影响。以2周龄的水稻幼苗为材料，分别进行水分亏缺（WD）、UV-B辐射（UV-B）以及3种不同的WD和UV-B组合处理：（I）WD和UV-B同时处理，（II）UV-B先于WD处理，（III）WD先于UV-B处理。用水势为-0.5MPa的聚乙二醇（PEG 6000）通气营养液浸泡根系，造成水分亏缺。冠层水平UV-B辐射剂量为24 kJ·m（-2）·d（-1）（0.84W·m（-2））。UV-B和WD单独和联合施加，导致，在一个时间依赖性的方式，增加SA含量在这两个机构。在WD胁迫植物中SA水平的增加伴随着PAL和BA 2 H活性的增加。然而，在植物暴露于UV-B的伴随下，只有BA 2 H的活性增加。在水分胁迫条件下，根中SA含量的增加早于叶，这可能表明SA参与了根与叶之间的信号传导。在WD和UV-B连续作用的植物中，无论其施加顺序如何，各单因素对叶片SA积累的影响均增强。WD对水分损失和膜伤害的影响大于UV-B辐射。植物经UV-B辐射预处理后再暴露于WD中，可观察到一种交叉耐受机制。这些植物的叶子没有表现出增加的脂质过氧化作用，测量的丙二醛含量，和水含量的减少。这种保护作用可能是由于在WD施加之前UV-B处理植物叶片中SA水平的增加引起的。(c)2012爱思唯尔有限公司版权所有。
The aim of the paper was to determine the effect of water deficit (WD) and UV-B radiation acting individually and in combination on salicylic acid (SA) accumulation as well as on the activity of phenylalanine ammonia-lyase (PAL) and benzoic acid hydroxylase (BA2H) that control its biosynthetic route from phenylalanine. An additional aim was to test whether the interaction of these stresses limits the negative effect of a single stress on tissue hydration and membrane injury. Two-week-old seedlings were subjected to water deficit (WD), UV-B irradiation (UV-B) and three different combinations of WD and UV-B: (I) WD and UV-B applied at the same time, (II) UV-B applied before WD, and (III) WD applied before UV-B. Water deficit was imposed by immersing the root system in aerated nutrient solution with polyethylene glycol (PEG 6000) of water potential - 0.5 MPa. UV-B dosage was 24 kJ m(-2) day(-1) (0.84 W m(-2)) at the canopy level. UV-B and WD imposed individually and jointly, caused, in a time-dependent manner, an increase in SA content in both organs. Increased levels of SA in WD stressed plants were accompanied by an increase in the activity of PAL and BA2H. However, in plants exposed to UV-B were accompanied only by an increase in the activity of BA2H. Under WD conditions, an earlier increase of SA content was observed in roots than in leaves, which may indicate the involvement of SA in the signal transduction between roots and leaves. In plants exposed to sequential action of WD and UV-B, regardless of the order of its imposition, the effect of each single factor on SA accumulation in leaves was strengthened. WD had a greater effect on water loss and membrane injury than UV-B radiation. In plants exposed to WD after pre-treatment with UV-B radiation, a cross-tolerance mechanism was observed. Leaves of these plants did not show increased lipid peroxidation, measured in terms of malondialdehyde content, and a decrease in water content. This protective action was probably caused by the increase of the SA level in leaves of the UV-B treated plants prior to WD imposition. (c) 2012 Elsevier B.V. All rights reserved.