Centrins control chicken cone cell lipid droplet dynamics through lipid-droplet-localized SPDL1.
Centrins control chicken cone cell lipid droplet dynamics through lipid-droplet-localized SPDL1.
复制标题
DOI：
10.1016/j.devcel.2023.08.012
复制
发表时间：
2023-09
影响因子：
11.8
通讯作者：
Huimin Pan;Yaqiang Qin;Jinglin Zhu;Wei Wang;Zhonghua Liu;Xiahe Huang;S. Lam;G. Shui;
中科院分区：
文献类型：
--
作者：
Huimin Pan;Yaqiang Qin;Jinglin Zhu;Wei Wang;Zhonghua Liu;Xiahe Huang;S. Lam;G. Shui;
作为进化上保守的细胞器，脂滴具有多种功能，并在不同的细胞类型和物种中具有不同的亚细胞定位。在鸟类锥体细胞中，有一个位于顶端的单一LD。我们证明了CIDEA(细胞死亡诱导DFFA样效应器a)和微管促进了鸡锥体细胞中单个LD的形成。Centrins是已知的中心粒蛋白，通过其C端钙结合结构域靶向锥体细胞LD。中心蛋白借助SPDL1-L(纺锤体卷曲蛋白1-L)定位于锥体细胞LDS，这是一种以前未描述的动粒相关动力蛋白适配器SPDL1的异构体。CETN3的缺失或CETN1的过表达会破坏锥体细胞LD的顶端定位。仿真分析表明，多个LD或单个错位LD会降低光敏度。总而言之，我们的发现确定了中心素在调节锥体细胞LD定位中的作用，这对锥体细胞的光敏性是重要的。
As evolutionarily conserved organelles, lipid droplets (LDs) carry out numerous functions and have various subcellular localizations in different cell types and species. In avian cone cells, there is a single apically localized LD. We demonstrated that CIDEA (cell death inducing DFFA like effector a) and microtubules promote the formation of the single LD in chicken cone cells. Centrins, which are well-known centriole proteins, target to the cone cell LD via their C-terminal calcium-binding domains. Centrins localize on cone cell LDs with the help of SPDL1-L (spindle apparatus coiled-coil protein 1-L), a previously uncharacterized isoform of the kinetochore-associated dynein adaptor SPDL1. The loss ofCETN3or overexpression of a truncated CETN1 abrogates the apical localization of the cone cell LD. Simulation analysis showed that multiple LDs or a single mispositioned LD reduces the light sensitivity. Collectively, our findings identify a role of centrins in the regulation of cone cell LD localization, which is important for the light sensitivity of cone cells.