Combined effects of growth factors and static mechanical compression on meniscus explant biosynthesis
Combined effects of growth factors and static mechanical compression on meniscus explant biosynthesis
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DOI：
10.1016/j.joca.2004.05.007
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发表时间：
2004-09-01
影响因子：
7
通讯作者：
Levenston, ME
中科院分区：
文献类型：
--
作者：
Imler, SM;Doshi, AN;Levenston, ME
目的：比较成纤维细胞生长因子-碱性（bFGF）、胰岛素样生长因子- i （IGF-I）、血小板衍生生长因子- ab （PDGF-AB）和转化生长因子- β 1 （tgf - β 1）对牛半月板组织外植体施加和不施加静态机械压迫的作用。设计：将半月板组织外植体在无血清环境中培养，并添加不同浓度的单个生长因子(1)培养4天，(2)在单一浓度下培养2-14天，(3)在单一浓度下培养4天，同时进行分级的静态压缩。用s -35-硫酸盐法和h -3-脯氨酸掺入法测定外植体中新合成的蛋白聚糖和总蛋白的积累情况。结果：在所选择的浓度范围内，tgf - β 1是蛋白质和蛋白多糖生产的最有效刺激物，而bFGF是最不有效的刺激物。在2周的时间里，所有四种生长因子对蛋白多糖产生的刺激持续存在，而在此期间没有对蛋白质产生的刺激。在每种合成代谢因子存在的情况下，静态机械压缩的叠加抑制了基质的产生，与未压缩的对照相比，对所有因子的抑制作用相当。结论：所选择的生长因子对半月板组织外植体具有合成代谢作用，促进基质的生成和沉积。静态机械压缩的加入对每种生长因子的基质生成产生了相当的相对抑制，这表明静态压缩和生长因子可能通过不同的途径调节半月板纤维软骨细胞的生物合成。(C) 2004国际骨关节炎研究学会。Elsevier Ltd.出版。版权所有。
Objective: To compare the actions of fibroblast growth factor-basic (bFGF), insulin-like growth factor-I (IGF-I), platelet derived growth factor-AB (PDGF-AB), and transforming growth factor-beta 1 (TGF-beta1) on bovine meniscus tissue explants with and without static mechanical compression.Design: Meniscus tissue explants were cultured in a serum-free environment supplemented with an individual growth factor (1) over a range of concentrations for 4 days, (2) at a single concentration for 2-14 days, and (3) at a single concentration for 4 days coupled with graded levels of static compression. Explants were analyzed for accumulation of newly synthesized proteoglycan and total protein as measured by S-35-sulfate and H-3-proline incorporation, respectively.Results: Over the range of chosen concentrations, TGF-beta1 was the most potent stimulator of both protein and proteoglycan production, whereas bFGF was the least effective stimulator. Over a 2-week period for all four growth factors, the stimulation of proteoglycan production was sustained while there was no stimulation of protein production during this period. The superposition of static mechanical compression inhibited matrix production in the presence of each anabolic factor, with comparable inhibition relative to uncompressed controls for all factors.Conclusions: The growth factors chosen exhibited an anabolic effect on the meniscus tissue explants, encouraging matrix production and deposition. The addition of static mechanical compression produced comparable relative inhibition of matrix production for each growth factor, suggesting that static compression and growth factors may modulate meniscal fibrochondrocyte biosynthesis via distinct pathways. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.