Bacillus subtilis and surfactin inhibit the transmissible gastroenteritis virus from entering the intestinal epithelial cells
Bacillus subtilis and surfactin inhibit the transmissible gastroenteritis virus from entering the intestinal epithelial cells
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枯草芽孢杆菌和表面活性素抑制传染性胃肠炎病毒进入肠上皮细胞
DOI：
10.1042/bsr20170082
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发表时间：
2017-04-30
影响因子：
4
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Wang, Xiaoqing;Hu, Weiwei;Yang, Qian
肠上皮细胞是传染性胃肠炎（TGE）病毒（TGEV）感染的靶点。迫切需要开发一种新的抗TGEV进入的候选药物。枯草芽孢杆菌是一种具有优良抑菌性能的益生菌，其分泌的表面素已被认为是对付大多数植物病原体，特别是包膜病毒的万能武器。本研究首次证实枯草芽孢杆菌OKB105及其表面素能有效抑制一种动物冠状病毒TGEV进入猪肠上皮细胞系（IPEC-J2）。然后，进行了几个不同的实验来寻求可能的机制。斑块分析表明，表面活性剂能以剂量依赖性的方式减少TGEV斑块的产生。同时，枯草芽孢杆菌与TGEV孵育1.5 h后，可以将TGEV颗粒附着在其表面，从而降低了与宿主细胞结合的病毒数量。此外，我们的数据表明，枯草芽孢杆菌的抑制与TGEV对病毒进入受体的竞争密切相关，包括表皮生长因子受体（EGFR）和氨肽酶N （APN）蛋白。此外，Western blotting和凋亡分析表明，枯草芽孢杆菌通过上调toll样受体(TLR)-6的表达，降低凋亡细胞的比例，增强IPEC-J2细胞的耐药性。综上所述，我们的研究结果表明，枯草芽孢杆菌OKB105及其表面素可以在体外拮抗TGEV的进入，并可能成为预防TGEV的有希望的新候选物。
Intestinal epithelial cells are the targets for transmissible gastroenteritis (TGE) virus (TGEV) infection. It is urgent to develop a novel candidate against TGEV entry. Bacillus subtilis is a probiotic with excellent anti-microorganism properties and one of its secretions, surfactin, has been regarded as a versatile weapon for most plant pathogens, especially for the enveloped virus. We demonstrate for the first time that B. subtilis OKB105 and its surfactin can effectively inhibit one animal coronavirus, TGEV, entering the intestinal porcine epithelial cell line (IPEC-J2). Then, several different experiments were performed to seek the might mechanisms. The plaque assays showed that surfactant could reduce the plaque generation of TGEV in a dose-dependent manner. Meanwhile, after incubation with TGEV for 1.5 h, B. subtilis could attach TGEV particles to their surface so that the number of virus to bind to the host cells was declined. Furthermore, our data showed that the inhibition of B. subtilis was closely related to the competition with TGEV for the viral entry receptors, including epidermal growth factor receptor (EGFR) and aminopeptidase N (APN) protein. In addition, Western blotting and apoptosis analysis indicated that B. subtilis could enhance the resistance of IPEC-J2 cells by up-regulating the expression of toll-like receptor (TLR)-6 and reducing the percentage of apoptotic cells. Taken together, our results suggest that B. subtilis OKB105 and its surfactin can antagonize TGEV entry in vitro and may serve as promising new candidates for TGEV prevention.