Ascl3 expression marks a progenitor population of both acinar and ductal cells in mouse salivary glands
Ascl3 expression marks a progenitor population of both acinar and ductal cells in mouse salivary glands
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DOI：
10.1016/j.ydbio.2008.04.018
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发表时间：
2008-08-01
影响因子：
2.7
通讯作者：
Ovitt, Catherine E.
中科院分区：
文献类型：
--
作者：
Bullard, Tara;Koek, Laurie;Ovitt, Catherine E.
Asc 13，也称为Sgn 1，是哺乳动物无毛细胞评分（Mash）转录因子基因家族的成员，其已经涉及细胞命运特化和分化。在小鼠唾液腺中，Ascl 3的表达限于导管细胞的子集。唾液腺的功能取决于负责唾液形成的分泌腺泡细胞和调节唾液并将其引导至口腔的导管细胞。唾液腺导管也是成体腺体中祖细胞的假定部位。使用Cre重组酶介导的报告系统，我们遵循了Ascl 3表达细胞的命运后，通过同源重组将EGFP-Cre表达盒引入Ascl 3基因座。谱系追踪显示，这些细胞是所有三个主要唾液腺中腺泡和导管细胞类型的祖细胞。在分化的后代中，Ascl 3的表达下调。这些数据直接证明了导管细胞和腺泡细胞隔室之间的祖细胞-子代关系，并鉴定了多能祖细胞群体，其以Ascl 3的表达为标志，其能够产生两种腺细胞类型。我们的结论是Ascl 3表达细胞有助于维持成人唾液腺。(C)2008年爱思唯尔公司All rights reserved.
Ascl3, also know as Sgn1, is a member of the mammalian achaete score (Mash) gene family Of transcription factors, which have been implicated in cell fate specification and differentiation. In the mouse salivary gland, expression of Ascl3 is restricted to a subset of duct cells. Salivary gland function depends on the secretory acinar cells, which are responsible for saliva formation, and duct cells, which modify the saliva and conduct it to the Oral cavity. The salivary gland ducts are also the putative site of progenitor cells in the adult gland. Using a Cre recombinase-mediated reporter system, we followed the fate of Ascl3-expressing cells after the introduction of an EGFP-Cre expression cassette into the Ascl3 locus by homologous recombination. Lineage tracing shows that these cells are progenitors of both acinar and ductal cell types in all three major salivary glands. In the differentiated progeny, expression of Ascl3 is down-regulated. These data directly demonstrate a progenitor-progeny relationship between duct cells and the acinar cell compartment, and identify a population of multipotent progenitor cells, marked by expression of Ascl3, which is capable of generating both gland cell types. We conclude that Ascl3-expressing cells contribute to the maintenance of the adult salivary glands. (C) 2008 Elsevier Inc. All rights reserved.