In vitro plasma protein adsorption on omega-functionalized alkanethiolate self-assembled monolayers
In vitro plasma protein adsorption on omega-functionalized alkanethiolate self-assembled monolayers
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DOI：
10.1021/la970187g
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发表时间：
1997-10-29
期刊：
影响因子：
3.9
通讯作者：
Tengvall, P
中科院分区：
文献类型：
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作者：
Lestelius, M;Liedberg, B;Tengvall, P
采用椭圆偏振法和抗体技术研究了烷硫醇盐在金表面的功能化自组装单分子膜（SAMs）对柠檬酸化人血浆蛋白的吸附。期望获得关于表面特性如何影响凝血和补体激活等事件的知识，这些事件反过来又影响植入部位的细胞激活。五个功能，甲基（-CH 3），三氟甲基酯（-O（C=O）CF 3），硫酸酯（-OSO 3 H），羧基（-COOH），羟基（-OH），进行了表征椭圆偏振法，接触角测量，扫描力显微镜（SFM），和傅里叶变换红外反射吸收光谱（IRAS）。低能量的表面，与甲基和三氟甲基酯的终端，表现出对纤维蛋白原的亲和力。三氟甲基酯也沉积脂蛋白（LP）。硫酸盐和羧基表面沉积凝血蛋白高分子量激肽原（HMWK），因子XII（F XII）和前激肽释放酶（PK），表明接触激活凝血。羟基官能化的SAM显示低沉积的血浆蛋白一般，虽然低结合的抗体对接触活化蛋白，补体因子3C（C3 C），和脂蛋白进行了观察。使用扫描力显微镜研究了形成的血浆蛋白层的形态。在低能量表面上，蛋白质倾向于聚集成大的结构。在甲基化的表面的情况下，形成了树枝状网络的外观。在高能表面上，蛋白质以小圆形簇的形式保持更均匀的分布。
Functionalized self-assembled monolayers (SAMs) of alkanethiolates on gold were used to study citrated human plasma protein adsorption by ellipsometry and antibody techniques in vitro. The aspiration is to gain knowledge about how surface properties affect such events like coagulation and complement activation, which in turn influence cell activation at an implant site. Five functionalities, methyl(-CH3), trifluoromethyl ester (-O(C=O)CF3), sulfate (-OSO3H), carboxyl (-COOH), and hydroxyl (-OH), were subject to characterization by ellipsometry, contact angle measurements, scanning force microscopy (SFM), and Fourier transform infrared reflection-absorption spectroscopy(IRAS). The low-energy surfaces, with methyl and trifluoromethyl ester terminations, showed affinity for fibrinogen. The trifluoromethyl ester also deposited lipoprotein (LP). The sulfate and the carboxyl surfaces deposited the coagulation proteins high molecular weight kininogen (HMWK), factor XII (F XII), and prekallikrein (PK), indicating contact activation of coagulation. Hydroxyl-functionalized SAMs showed low deposition of plasma proteins in general, although a low binding of antibodies against the contact activation proteins, complement factor 3c (C3c), and lipoproteins was observed. The morphology of the formed plasma protein layers was studied using scanning force microscopy. On the low-energy surfaces the proteins tended to cluster into large formations. In the case of the methylated surface the formations had the appearance of dendrite-like networks. On the high-energy surfaces the proteins retained a more uniform spreading in small rounded clusters.