The protein expression profile of ACE2 in human tissues
The protein expression profile of ACE2 in human tissues
复制标题
DOI：
10.15252/msb.20209610
复制
发表时间：
2020-07-01
影响因子：
9.9
通讯作者：
Lindskog, Cecilia
中科院分区：
文献类型：
--
作者：
Hikmet, Feria;Mear, Loren;Lindskog, Cecilia
新型SARS冠状病毒2（SARS-CoV-2）对医疗保健和社会构成了全球性挑战。为了了解SARS-CoV-2感染的易感性，宿主细胞表面受体的细胞类型特异性表达是必要的。被认为参与宿主细胞进入的关键蛋白是血管紧张素I转化酶2（ACE 2）。在这里，我们报告了ACE 2在超过150种不同细胞类型中的表达模式，这些细胞类型对应于所有主要的人体组织和器官，基于严格的免疫组织化学分析。将结果与mRNA和蛋白质水平上的几个数据集进行比较。ACE 2表达主要见于肠上皮细胞、肾小管、胆囊、心肌细胞、雄性生殖细胞、胎盘滋养层细胞、导管细胞、眼和血管系统。在呼吸系统中，表达是有限的，在少数个体的细胞亚群中没有表达或仅低表达，仅通过一种抗体观察到。我们的数据构成了进一步研究SARS-CoV-2宿主细胞进入的重要资源，以了解该疾病的生物学，并帮助开发有效的病毒感染治疗方法。
The novel SARS-coronavirus 2 (SARS-CoV-2) poses a global challenge on healthcare and society. For understanding the susceptibility for SARS-CoV-2 infection, the cell type-specific expression of the host cell surface receptor is necessary. The key protein suggested to be involved in host cell entry is angiotensin I converting enzyme 2 (ACE2). Here, we report the expression pattern of ACE2 across > 150 different cell types corresponding to all major human tissues and organs based on stringent immunohistochemical analysis. The results were compared with several datasets both on the mRNA and protein level. ACE2 expression was mainly observed in enterocytes, renal tubules, gallbladder, cardiomyocytes, male reproductive cells, placental trophoblasts, ductal cells, eye, and vasculature. In the respiratory system, the expression was limited, with no or only low expression in a subset of cells in a few individuals, observed by one antibody only. Our data constitute an important resource for further studies on SARS-CoV-2 host cell entry, in order to understand the biology of the disease and to aid in the development of effective treatments to the viral infection.