The Effect of ADAM8 on the Proliferation and Apoptosis of Hepatocytes and Hepatoma Carcinoma Cells
The Effect of ADAM8 on the Proliferation and Apoptosis of Hepatocytes and Hepatoma Carcinoma Cells
复制标题
DOI：
10.1002/jbt.21737
复制
发表时间：
2015-09-01
影响因子：
3.6
通讯作者：
Lu, Hua-Jie
中科院分区：
文献类型：
--
作者：
Li, San-Qiang;Hu, Zhi-Hong;Lu, Hua-Jie
本研究旨在探讨ADAM8在肝细胞癌进展过程中对肝细胞和肝癌细胞增殖和凋亡的影响。在小鼠肝细胞癌发生发展过程中，ADAM8的表达明显增加，并与增殖细胞核抗原表达增加和细胞凋亡减少有良好的相关性。体外增殖实验表明，重组ADAM8能诱导L02细胞表达增殖细胞核抗原，但不能诱导HepG2细胞表达增殖细胞核抗原。体外细胞凋亡实验表明，重组ADAM8不诱导或抑制L02细胞中凋亡相关因子Bcl2、Bax和Caspase3的表达，但显著诱导HepG2细胞中Bcl2的表达，抑制Bax和Caspase3的表达。综上所述，我们的研究提示ADAM8可以促进正常肝细胞的增殖，使肝癌细胞具有更强的抗凋亡能力，在肝细胞癌的发生发展过程中发挥重要作用。ADAM8；增殖；凋亡
This study was undertaken to evaluate the effect of ADAM8 on the proliferation and apoptosis of hepatocytes and hepatoma carcinoma cells during hepatocellular carcinoma (HCC) progression. The expression of ADAM8 was significantly increased with good correlation of PCNA expression increasing and cells apoptosis decreasing during the progression of HCC in the liver of mice. Proliferation experiment in vitro showed that recombinant ADAM8 could induce the expression of PCNA in L02 cells, but not in HepG2 cells. Apoptosis experiment in vitro showed that recombinant ADAM8 did not induce or inhibit the expression of apoptosis-related factors Bcl2, Bax, and Caspase3 in L02 cells, but significantly induced the expression of Bcl2, inhibited the expression of Bax and Caspase3 in HepG2 cells. In conclusion, our study suggested that ADAM8 could promote the proliferation of normal hepatocytes and render hepatoma carcinoma cells more resistant to apoptosis to play important roles during the progression of HCC. ADAM8; Proliferation; Apoptosis