Liquid chromatography/mass-spectrometric characterization of sphingomyelin and dihydrosphingomyelin of human lens membranes.
Liquid chromatography/mass-spectrometric characterization of sphingomyelin and dihydrosphingomyelin of human lens membranes.
复制标题
人晶状体膜的鞘磷脂和二氢鞘磷脂的液相色谱/质谱表征。
DOI：
10.1159/000268014
复制
发表时间：
1997
影响因子：
2.1
通讯作者：
Borchman,D
中科院分区：
文献类型：
--
作者：
Byrdwell,WC;Borchman,D
The identity of the major phospholipid component of human lens membrane extracts, previously referred to as the ‘unknown phospholipid’, was recently proposed to be 4,5-dihydrosphingomyelin (DHS). Using high-performance liquidchromatographic separation with atmospheric pressure chemical ionization and electrospray ionization mass-spectrometric detection, we report here the first identification of the molecular species of DHSs and sphingomyelins (SPMs) of the human eye lens. The most abundant molecular species were palmitic and tetracosenoic (24:1) DHSs, representing 57.8 and 23.3% of human lens DHSs, respectively. Lesser amounts ofhexacosanoic, hexacosenoic, tetracosanoic, docosanoic, docosenoic, stearic, palmitoleic, myristic and other DHSs were found. The most abundant normal SPM molecular species in the human lens were similar to those of DHS. Palmitic SPM represented 53.9% of the uncorrected SPM peak areas, while tetracosenoic SPM represented 17.6% of the SPM in the human lens. The sphingolipids of the human lens were determined to be composed of 76.9% DHS species and 23.1% SPM species. Commercially available SPM was also found to contain significant amounts of DHS species.
