The relationship between infiltrating CD4+ lymphocytes, activated eosinophils, and the magnitude of the allergen-induced late phase cutaneous reaction in man.
The relationship between infiltrating CD4+ lymphocytes, activated eosinophils, and the magnitude of the allergen-induced late phase cutaneous reaction in man.
复制标题
浸润性 CD4 淋巴细胞、活化的嗜酸性粒细胞与人类过敏原诱导的晚期皮肤反应的程度之间的关系。
作者：
A. Frew;A. Kay
用一组单抗，用免疫细胞化学方法研究了人晚期变态反应性皮肤反应中白细胞的表型和活化状态。用变应原提取物(草花粉或屋尘螨)皮内激发特应性个体的晚期皮肤反应，并在激发后6、24或48h取材。冰冻切片检查T细胞和嗜酸性粒细胞的渗透和激活。来自盐水挑战的对照部位的活检用于比较。在真皮毛细血管附近可见大量CD3+细胞。CD_4~+细胞的数量与之相似，但CD8~+细胞很少。外周血中CD_4/CD_8比值与组织中CD_4/CD_8比值无关。有两个观察结果表明T细胞已经被激活：一是少数浸润性细胞携带IL-2受体，二是有间接证据表明有干扰素-γ的分泌，表现为内皮细胞上人类白细胞抗原-DR的表达增加和表皮朗格汉斯细胞上的CD4Ag的从头表达。在大多数过敏原挑战的活检组织中都检测到活化的嗜酸性粒细胞。6h时的晚期反应直径与48h时活化的嗜酸性粒细胞数呈正相关(r=0.61，p=0.05)，而与T细胞的浸润无关。24 h时CD_4~+细胞数与活化的嗜酸性粒细胞数呈正相关(r=0.94，p<0.001)。这些发现表明，T淋巴细胞和嗜酸性粒细胞之间的相互作用在对空气传播的过敏原的反应中可能比之前认识到的更重要。
The phenotype and activation status of leukocytes infiltrating human late phase allergic skin reactions were investigated by immunocytochemistry using a panel of mAb. Late phase skin reactions were induced in atopic individuals by intradermal challenge with allergen extract (grass pollen or house dust mite) and skin biopsies were obtained 6, 24, or 48 h after challenge. Cryostat sections were examined for evidence of infiltration and activation of T cells and eosinophils. Biopsies from saline-challenged control sites were used for comparison. A substantial number of CD3+ cells were observed close to the dermal capillaries. The number of CD4+ cells showed a similar pattern but few CD8+ cells were seen. The ratio of CD4 and CD8 subsets in tissue did not relate to the CD4/CD8 ratio in the peripheral blood. Two observations suggested that T cells had become activated: first, a small number of infiltrating cells bore receptors for IL-2, and second, there was indirect evidence of IFN-gamma secretion, demonstrated by increased expression of HLA-DR on endothelial cells and de novo expression of CD4 Ag on epidermal Langerhans cells. Activated eosinophils were detected in most of the allergen-challenged biopsies. The late phase reaction diameter at 6 h correlated with the number of activated eosinophils at 48 h (r = 0.61, p = 0.05), but not with infiltration by T cells. There was a strong correlation between the numbers of CD4+ cells and activated eosinophils at 24 h (r = 0.94, p less than 0.001). These findings suggest that interactions between T lymphocytes and eosinophils in reactions to airborne allergens may be more important than has previously been recognized.