Expression of protein 4.1G in Schwann cells of the peripheral nervous system
Expression of protein 4.1G in Schwann cells of the peripheral nervous system
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DOI：
10.1002/jnr.20949
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发表时间：
2006-08-15
影响因子：
4.2
通讯作者：
Ohno, Shinichi
中科院分区：
文献类型：
--
作者：
Ohno, Nobuhiko;Terada, Nobuo;Ohno, Shinichi
包括蛋白4.1家族在内的细胞骨架蛋白在细胞膜完整性、蛋白靶向和信号转导等方面发挥着重要作用。虽然蛋白4.1G（4.1G）在哺乳动物组织中普遍表达，但它在细胞内可以具有非常离散的分布。本研究观察了4.1G在大鼠坐骨神经中的表达和分布。北方和Western blot分析显示，大鼠坐骨神经提取液中含有丰富的4.1G mRNA和蛋白。用4.1G特异性抗体进行免疫组织化学染色，并用E-钙粘蛋白、β IV血影蛋白和连接蛋白32进行双重免疫标记，在结旁环、Schmidt-Lanterman切口、啮齿动物坐骨神经的轴周膜、轴中膜和轴下膜中检测到4.1G。免疫电镜证实了4.1G在雪旺细胞中的免疫分布。在发育中的小鼠坐骨神经中，4.1G弥漫分布在未成熟的雪旺细胞中，并在其成熟过程中逐渐定位于副阳极，切口，轴周膜和中轴突膜。这些数据支持的概念，4.1 G在膜扩张和专业化，发生在周围神经系统中的髓鞘节间的形成和维持过程中发挥重要作用。(c)2006 Wiley-Liss，Inc.
The membrane-associated cytoskeletal proteins, including protein 4.1 family, play important roles in membrane integrity, protein targeting, and signal transduction. Although protein 4.1G (4.1G) is expressed ubiquitously in mammalian tissues, it can have very discrete distributions within cells. The present study investigated the expression and distributions of 4.1G in rodent sciatic nerve. Northern and Western blot analysis detected abundant 4.1G mRNA and protein in rat sciatic nerve extracts. Immunohistochemical staining with a 4.1G-specific antibody and double immunolabeling with E-cadherin, beta IV spectrin, and connexin 32 detected 4.1G in paranodal loops, Schmidt-Lanterman incisures, and periaxonal, mesaxonal, and abaxonal membranes of rodent sciatic nerve. Immunoelectron microscopy confirmed the immunodistribution of 4.1G in Schwann cells. In developing mouse sciatic nerves, 4.1G was diffusely distributed in immature Schwann cells and gradually localized at paranodes, incisures, and periaxonal and mesaxonal membranes during their maturation. These data support the concept that 4.1 G plays an important role in the membrane expansion and specialization that occurs during formation and maintenance of myelin internodes in the peripheral nervous system. (c) 2006 Wiley-Liss, Inc.