Expression of a novel NAC domain-containing transcription factor (CaNAC1) is preferentially associated with incompatible interactions between chili pepper and pathogens
Expression of a novel NAC domain-containing transcription factor (CaNAC1) is preferentially associated with incompatible interactions between chili pepper and pathogens
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DOI：
10.1007/s00425-005-0030-1
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发表时间：
2005-11-01
期刊：
影响因子：
4.3
通讯作者：
Choi, D
中科院分区：
文献类型：
--
作者：
Oh, SK;Lee, S;Choi, D
我们的目的是分离基因辣椒的超敏反应过程中的非宿主病原体感染的调控，以期阐明对病原体攻击的防御反应。在90个转录因子的初步特征在于通过反向RNA凝胶印迹分析，一个cDNA克隆，CaNAC 1（辣椒NAC 1）含有植物特异性NAC结构域基序的进一步表征。在辣椒与细菌或病毒病原体之间的不相容相互作用期间，快速特异地诱导CaNAC 1基因的表达。此外，外源水杨酸和乙烯利对该基因有强烈的诱导作用，而茉莉酸甲酯的诱导作用是短暂的。CaNAC 1-smGFP融合蛋白转染洋葱表皮后定位于细胞核。使用酵母系统，我们进一步揭示了CaNAC 1的转录激活结构域位于蛋白质的C-末端的一半。我们的研究结果共同表明，植物特异性NAC结构域蛋白，CaNAC 1，可能在植物防御反应的调节中发挥作用。
We aim to isolate genes in chili pepper that are regulated during the hypersensitive response to infection by nonhost pathogens, with a view to elucidating the defense responses against pathogen attack. Among the 90 transcription factors initially characterized by reverse RNA gel blot analysis, a cDNA clone, CaNAC1 (Capsicum annuum NAC1) containing the plant-specific NAC domain motif was further characterized. Expression of the CaNAC1 gene was rapidly and specifically induced during incompatible interactions between pepper and bacterial or viral pathogens. Additionally, this gene was strongly induced by exogenously applied salicylic acid and ethephon, whereas methyl jasmonate only had a transient effect. A CaNAC1-smGFP (soluble modified green fluorescent protein) fusion protein localized to the nucleus following transfection into the epidermis of onion. Using the yeast system, we further disclose that the transcription activation domain of CaNAC1 is located in the C-terminal half of the protein. Our results collectively suggest that the plant-specific NAC domain protein, CaNAC1, may play a role in the regulation of defense responses in plants.