Aged garlic extract and its constituents inhibit platelet aggregation through multiple mechanisms
Aged garlic extract and its constituents inhibit platelet aggregation through multiple mechanisms
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DOI：
10.1093/jn/136.3.782s
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发表时间：
2006-03-01
影响因子：
4.2
通讯作者：
Rahman, K
中科院分区：
文献类型：
--
作者：
Allison, GL;Lowe, GM;Rahman, K
陈蒜提取物 (AGE) 是一种复杂的混合物。其成分包括蒜氨酸、环蒜氨酸、S-烯丙基-L-半胱氨酸、S-甲基-L-半胱氨酸、S-乙基半胱氨酸、S-1-丙酰-L-半胱氨酸、S-烯丙基硫基-L-半胱氨酸、果糖基-精氨酸和e-绿原素。它还含有L-精氨酸、L-半胱氨酸和L-蛋氨酸。 AGE 降低与心血管疾病相关的参数，包括抑制血小板聚集。然而，潜在的机制尚未确定，这是本研究的主题。 AGE 以浓度依赖性方式抑制血小板聚集，并且在 1.56-25% (v:v) 浓度之间达到显着效果。当以混合物形式测试 AGE 的成分时，显示出双相抑制模式，尽管这并不显着。 L-蛋氨酸在 0.00078、0.00625 和 0.1 mmol/L 浓度下表现出抗聚集特性，而 L-精氨酸和 L-半胱氨酸在 9 mmol/L 浓度下有效。然而，当这些成分和氨基酸一起测试时，仅在1 mmol/L的浓度下观察到对血小板聚集的显着抑制。 AGE 在 12.5% 和 25% (v:v) 浓度下也表现出分解特性。当作为混合物进行测试时，这些成分和氨基酸在浓度为 0.25 和 1 mmol/L 时表现出分解特性。相比之下，AGE 的乙醚提取物对血小板聚集没有影响。当用 A23187 或 ADP 刺激血小板时，血小板内 Ca2+ 的增加伴随着血小板聚集。 Ca2+ 的增加在 AGE 存在下被消除。 AGE 很可能以协同方式发挥作用，并对血小板聚集所涉及的生化途径产生多种影响。
Aged garlic extract (AGE) is a complex mixture. Its constituents include allin, cycloalliin, S-allyl-L-cysteine, S-methyl-L-cysteine, S-ethylcysteine, S-1-proponyl-L-cysteine, S-allylmercapto-L-cysteine, fructosyl-arginine, and,e-chlorogenin. It also contains L-arginine, L-cysteine, and L-methionine. AGE reduces the parameters associated with cardiovascular disease, including the inhibition of platelet aggregation. However, the underlying mechanisms have yet to be established and are the subject of this study. AGE inhibited platelet aggregation in a concentration-dependent manner, and this achieved significance between concentrations of 1.56-25% (v:v). The constituents of AGE, when tested as a mixture, displayed a biphasic pattern of inhibition, although this was not significant. L-Methionine displayed anti-aggregatory properties at concentrations of 0.00078, 0.00625, and 0.1 mmol/L, whereas L-arginine and L-cysteine were effective at 9 mmol/L. However, when the constituents and the amino acids were tested together, significant inhibition of platelet aggregation was observed only at a concentration of 1 mmol/L. AGE also displayed disaggregatory properties at concentrations of 12.5 and 25% (v:v). The constituents and the amino acids, when tested as a mixture, displayed disaggregatory properties at concentrations of 0.25 and 1 mmol/L. In contrast, a diethyl-ether extract of AGE had no effect on platelet aggregation. When platelets were stimulated with either A23187 or ADP, an increase in intraplatelet Ca2+ accompanied platelet aggregation. This increase in Ca2+ was abolished in the presence of AGE. It is likely that AGE works in a synergistic manner and exerts multiple effects on the biochemical pathways involved in platelet aggregation.