Allicin inhibits transient outward potassium currents in mouse ventricular myocytes
Allicin inhibits transient outward potassium currents in mouse ventricular myocytes
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DOI：
10.3892/etm.2016.3116
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发表时间：
2016-05-01
影响因子：
2.7
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Cao, Hong;Huang, Congxin;Wang, Xin
大蒜素是大蒜的活性成分，大蒜是一种广泛使用的香料和食物。大蒜的治疗作用也得到了广泛的研究，已证明大蒜素能够抑制心房肌细胞的瞬时外向钾电流(I-to)。然而，大蒜素对心肌细胞I-TO的直接作用尚不清楚。采用全细胞膜片记录技术，观察了大蒜素对小鼠心室肌细胞I-TO的影响。结果表明，大蒜素低剂量组(10mU/L，P>；0.05)对Ito电流无明显抑制作用。但较高剂量的大蒜素(30、100和300mU/L；P=100mU/L)可显著抑制小鼠心肌细胞I-TO的激活，并能加速电压依赖性失活。综上所述，本研究揭示了大蒜素抑制小鼠心室肌细胞I-TO的作用，这可能是大蒜素发挥抗心律失常作用的机制。
Allicin is the active constituent of garlic, a widely used spice and food. The remedial properties of garlic have also been extensively researched and it has been demonstrated that allicin is able to inhibit the transient outward potassium current (I-to) in atrial myocytes. However, the direct effect of allicin on I-to in ventricular myocytes has yet to be elucidated. In the present study, the effects of allicin on I-to in ventricular myocytes isolated from mice were investigated, using the whole-cell patch recording technique. The results revealed that Ito current was not significantly suppressed by allicin in the low-dose group (10 mu mol/l; P>0.05). However, Ito was significantly inhibited by higher doses of allicin (30, 100 and 300 mu mol/l; P= 100 mu mol/l) was able to accelerate the voltage-dependent inactivation of I-to in mouse ventricular myocytes. In conclusion, the present study revealed that allicin inhibited the I-to in mouse ventricular myocytes, which may be the mechanism through which allicin exerts its antiarrhythmic effect.