Fimbrial lectins influence the chemokine repertoire in the urinary tract mucosa
Fimbrial lectins influence the chemokine repertoire in the urinary tract mucosa
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DOI：
10.1038/sj.ki.5002076
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发表时间：
2007-04-01
影响因子：
19.6
通讯作者：
Svanborg, C.
中科院分区：
文献类型：
--
作者：
Godaly, G.;Otto, G.;Svanborg, C.
对粘膜感染的防御依赖于趋化因子，其将炎性细胞募集到粘膜。本研究检测了趋化因子对尿路致病性大肠杆菌的反应是否受菌毛表达的影响。体外感染膀胱上皮细胞后，定量检测CXC（CXCL 1、CXCL 5、CXCL 8、CXCL 9、CXCL 10）和CC趋化因子（CCL 2、CCL 3、CCL 5）。coli肾盂肾炎分离株，或与无毒E. coli K-12菌株。反应曲线随菌毛类型而变化。1型菌毛E. coli主要诱导CXCL 1和CXCL 8的表达，而P则诱导E.大肠杆菌刺激的CCL 2和CCL 5和II类趋化因子诱导剂比III类P菌毛更有效。还对73例发热性尿路感染患者的尿液样本中的趋化因子进行了定量，并分析了感染每位患者的菌株的疾病严重程度和菌毛表达的函数。在患者尿液中检测到复杂的CXC和CC趋化因子反应，菌毛类型具有显著影响。结果表明，毒力因子如菌毛可能会改变粘膜趋化因子的反应。这种机制可能允许宿主调节炎性细胞浸润以适应感染菌株。
The defense against mucosal infections relies on chemokines that recruit inflammatory cells to the mucosa. This study examined if the chemokine response to uro-pathogenic Escherichia coli is influenced by fimbrial expression. The CXC (CXCL1, CXCL5, CXCL8, CXCL9, CXCL10) and CC chemokines (CCL2, CCL3, CCL5) were quantified after in vitro infection of uro-epithelial cells with a fimbriated E. coli pyelonephritis isolate, or with P or type 1 fimbriated transformants of an avirulent E. coli K-12 strain. The response profile was shown to vary with the fimbrial type. Type 1 fimbriated E. coli elicited mainly CXCL1 and CXCL8, whereas P fimbriated E. coli stimulated CCL2 and CCL5 and class II were more potent chemokine inducers than class III P fimbriae. Chemokines were also quantified in urine samples from 73 patients with febrile urinary tract infection, and analyzed as a function of disease severity and fimbrial expression by the strain infecting each patient. A complex CXC and CC chemokine response was detected in patient urine, with a significant influence of the fimbrial type. The results show that virulence factors like fimbriae may modify the mucosal chemokine response. This mechanism may allow the host to adjust the inflammatory cell infiltrate to fit the infecting strain.