Prevalence and Clinical Impact of Human Pegivirus-1 Infection in HIV-1-Infected Individuals in Yunnan, China.
Prevalence and Clinical Impact of Human Pegivirus-1 Infection in HIV-1-Infected Individuals in Yunnan, China.
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中国云南省 HIV-1 感染者中人类 Pegivirus-1 感染的患病率和临床影响
DOI：
10.3390/v9020028
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发表时间：
2017-02-15
期刊：
影响因子：
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通讯作者：
Xia X
中科院分区：
文献类型：
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作者：
Miao Z;Gao L;Song Y;Yang M;Zhang M;Lou J;Zhao Y;Wang X;Feng Y;Dong X;Xia X
人类Pegivirus-1（HPgV-1）可能对人类免疫缺陷病毒-1（HIV-1）感染的疾病进展产生有益影响。然而，HPgV-1的基因型多样性及其与HIV-1疾病进展的相关性的分析仍然有限。在云南省16个地（州）共招募了1062名HIV-1感染者。采用逆转录巢式聚合酶链反应（RT-nPCR）、系统发育分析和临床资料分析方法检测HPgV-1感染，确定基因型，分析HPgV-1基因型对HIV-1疾病进展的影响。HPgV-1 RNA总阳性率为23.4%（248/1062），注射吸毒者HPgV-1感染率（28.5%，131/460）明显高于异性恋者（19.4%，117/602）。在212例成功测序E2基因的受试者中鉴定出多种基因型，包括基因型7（55.7%）、基因型3（34.9%）、基因型4（4.7%）、基因型2（3.3%）和未分类组（1.4%）。此外，基因型7在注射吸毒者中占主导地位，而基因型3在异性恋者中最常见。我们的研究结果显示，HPgV-1基因型7组表现出显着较低的HIV-1病毒载量和较高的CD 4+细胞计数。这一发现表明HPgV-1基因型7可能与HIV-1疾病的更好进展相关。
Human Pegivirus-1 (HPgV-1) may have a beneficial impact on disease progression in human immunodeficiency virus-1 (HIV-1) infection. However, analysis of the genotypic diversity of HPgV-1 and its relevance to the progression of HIV-1 disease remains limited. A total of 1062 HIV-1-infected individuals were recruited in all sixteen prefectures of Yunnan province, China. The reverse transcription nested polymerase chain reaction (RT-nPCR), phylogenetic analyses, and clinical data analyses were used to detect HPgV-1 infection, determine genotype, and analyze HPgV-1 genotype impact on HIV-1 disease progression. The overall positive rate of HPgV-1 RNA was 23.4% (248/1062), and the frequency of HPgV-1 infection in injecting drug users (IDUs) (28.5%, 131/460) was significantly higher than in heterosexuals (19.4%, 117/602). Multiple genotypes were identified in 212 subjects with successful sequencing for the E2 gene, including genotype 7 (55.7%), genotype 3 (34.9%), genotype 4 (4.7%), genotype 2 (3.3%), and an unclassified group (1.4%). Moreover, genotype 7 predominated in IDUs, whereas genotype 3 was the most common in heterosexuals. Our results revealed that HPgV-1 genotype 7 groups exhibited significantly lower HIV-1 viral load and higher CD4+ cell counts. This finding suggests that HPgV-1 genotype 7 may be associated with a better progression of HIV-1 disease.