Neurite outgrowth-regulating properties of GABA and the effect of serum on mouse spinal cord neurons in culture
Neurite outgrowth-regulating properties of GABA and the effect of serum on mouse spinal cord neurons in culture
复制标题
DOI：
10.1046/j.1469-7580.1998.19340503.x
复制
发表时间：
1998-11-01
影响因子：
2.4
通讯作者：
Owen, A
中科院分区：
文献类型：
--
作者：
Bird, M;Owen, A
采用延时摄影法观察γ -氨基丁酸（GABA）对小鼠脊髓培养神经突生长和运动的影响。100、10和1 μ M GABA显著抑制了神经突的生长，100和10 μ M GABA显著降低了生长锥的运动性。GABA(A)受体激动剂巴氯芬可以模拟这种效果，而GABA(A)受体激动剂muscimol则没有效果。GABA对生长和运动的影响似乎取决于所使用的血清类型。本文报告的结果仅在使用热灭活血清时获得，而在培养基中加入非热灭活血清时则没有。他们认为GABA在小鼠胚胎脊髓的生长过程中起着调节作用。
Time-lapse photography was used to examine the effects of gamma-aminobutyric acid (GABA) on the outgrowth and motility of neurites in cultures from mouse spinal cord. GABA at concentrations of 100, 10 and 1 mu M caused significant inhibition of neurite outgrowth and the motility of growth cones was significantly reduced by treatment with 100 and 10 mu M GABA. This effect was mimicked by the GABA(B) receptor agonist baclofen, whereas the GABA(A) receptor agonist muscimol had no effect. The effect of GABA on outgrowth and motility seems to be dependent on the type of serum employed. The results reported here were obtained only when heat-inactivated serum was used and not when non heat-inactivated serum was added to the culture medium. They suggest that GABA has a role in the regulation of process outgrowth within the embryonic mouse spinal cord.