Silicon nanowires-based highly-efficient SERS-active platform for ultrasensitive DNA detection
Silicon nanowires-based highly-efficient SERS-active platform for ultrasensitive DNA detection
复制标题
基于硅纳米线的高效 SERS 活性平台，用于超灵敏 DNA 检测
DOI：
10.1016/j.nantod.2011.02.004
复制
发表时间：
2011-04-01
期刊：
影响因子：
17.4
通讯作者：
Lee, Shuit-Tong
中科院分区：
文献类型：
--
作者：
He, Yao;Su, Shao;Lee, Shuit-Tong
一种纳米结构的复合物，即原位生长的AgNPs包覆的硅纳米线（SiNWs@AgNPs），被开发为一种定义明确的表面增强拉曼散射（Sers）活性平台，用于超灵敏的DNA检测。这种SiNWsAgNPs纳米结构具有类似于10（10）的极高Sers增强因子。我们设计了一个DNA传感器的基础上的纳米结构，通过固定的捕获探针DNA在表面的银纳米粒子，和一个三明治的策略，用于检测目标DNA，使一个报告探针标记的染料的表面附近，导致高Sers信号。这种基于硅纳米结构的生物传感器平台可以检测到非常低的DNA浓度，接近1 fM，这与通过Sers检测到的最低DNA浓度相当。我们期望这种高灵敏度和鲁棒性的基于SiNWs的Sers平台可以作为生物分子传感和生物医学分析的实用和强大的工具。(C)2011爱思唯尔有限公司保留所有权利。
A nanostructured complex, silicon nanowires (SiNWs) coated with in situ grown AgNPs (SiNWs@AgNPs), is developed as a well-defined surface-enhanced Raman Scattering (SERS)-active platform for ultrasensitive DNA detection. Such SiNWs@AgNPs nanostructure possesses an extremely high SERS enhancement factor of similar to 10(10). We design a DNA sensor based on the nanostructure via immobilization of capture probe DNA at the surface of AgNPs, and a sandwich strategy for the detection of target DNA that brings a reporter probe labeled with a dye to the proximity of the surface, leading to high SERS signals. This silicon nanostructure-based biosensor platform can detect a remarkably low DNA concentration at similar to 1 fM, which is comparable to the lowest DNA concentration ever detected via SERS. We expect this highly sensitive and robust SiNWs-based SERS platform may serve as a practical and powerful tool for biomolecular sensing and biomedical analysis. (C) 2011 Elsevier Ltd. All rights reserved.