Chiral recognition of deoxyoligonucleotides by delta- and lambda-tris(ethylenediamine)cobalt(III).
Chiral recognition of deoxyoligonucleotides by delta- and lambda-tris(ethylenediamine)cobalt(III).
复制标题
DOI：
10.1021/bi00043a011
复制
发表时间：
1995-10
期刊：
影响因子：
2.9
通讯作者：
Q. Xu;S. Jampani;H. Deng;W. Braunlin
中科院分区：
文献类型：
--
作者：
Q. Xu;S. Jampani;H. Deng;W. Braunlin
59Co NMR 和 CD 测量结果表明，Co(en)3(3+) 的两种立体异构体在 DNA 识别的序列依赖性方面具有相似的趋势，正如之前针对 Co(NH3)6(3+) 报道的那样。特别地，对于具有两个或更多个同链鸟嘌呤残基的DNA分子，特异性结合是明显的。任一异构体与此类序列的结合都会诱导向 A-DNA 特征的结构转变。此类测量还显示了两种立体异构体在识别特定双链 DNA 序列的方式方面存在显着差异。 δ-Co(en)3(3+) 比 lambda-Co(en)3(3+) 与富含鸟嘌呤的右旋 DNA 结合更紧密，而 lambda-Co(en)3(3+) 比 delta-Co(en)3(3+) 与左旋 DNA 结合更紧密。这两种立体异构体以无法区分的方式与富含 AT 的 DNA 结合。
59Co NMR and CD measurements show for both stereoisomers of Co(en)3(3+) a similar trend in the sequence dependence of DNA recognition, as was reported previously for Co(NH3)6(3+). In particular, specific binding is evident to DNA molecules possessing runs of two or more same-strand guanine residues. The binding of either isomer to such sequences induces structural transitions toward A-DNA characteristics. Such measurements also show significant differences between the two stereoisomers in terms of how they recognize specific duplex DNA sequences. delta-Co(en)3(3+) binds more tightly than lambda-Co(en)3(3+) to right-handed, guanine-rich DNA, whereas lambda-Co(en)3(3+) binds more tightly than delta-Co(en)3(3+) to left-handed DNA. The two stereoisomers bind in an indistinguishable manner to AT-rich DNA.