Multiple sclerosis: II. Effects of prothymosin alpha on the autologous and allogeneic MLR in patients with multiple sclerosis.
Multiple sclerosis: II. Effects of prothymosin alpha on the autologous and allogeneic MLR in patients with multiple sclerosis.
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多发性硬化症：II。
DOI：
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发表时间：
1987
影响因子：
4.6
通讯作者：
M. Papamichail
中科院分区：
文献类型：
--
作者：
G. Reclos;C. Baxevanis;C. Sfagos;C. Papageorgiou;G. Tsokos;M. Papamichail
我们最近发现多发性硬化（MS）患者外周血单核细胞在刺激自体和同种异体T淋巴细胞方面存在缺陷。发现这种缺陷与疾病活动相关。在这份报告中，我们证明了大鼠胸腺组分5多肽--胸腺素原α（ProT α）可以恢复MS单核细胞刺激缺陷。当使用活动性疾病患者的单核细胞作为刺激物时，诱导混合淋巴细胞反应（MLR）最佳增强的ProT α浓度显著高于使用非活动性疾病患者的单核细胞时。用从MS的非活动期收获的自体刺激性单核细胞测试的T4+细胞表现出比用从急性复发获得的自体单核细胞刺激时高得多的增殖反应。MS患者中T4+细胞的自体增殖降低在与ProT α在自体单核细胞的环境中预孵育后恢复到正常水平。我们的研究结果表明，ProT α能够完全恢复MS单核细胞的刺激功能缺陷和MS衍生的T4+细胞的单核细胞相关功能缺陷。
We have recently demonstrated that peripheral blood monocytes from patients with multiple sclerosis (MS) have a defect in stimulating autologous and allogeneic T lymphocytes. This defect was found to correlate with disease activity. In this report we demonstrate that prothymosin alpha (ProT alpha), a rat thymus fraction 5 polypeptide, restores the MS monocyte stimulatory defect. The concentrations of ProT alpha which induced optimal enhancement of the mixed lymphocyte responses (MLR) were significantly higher when monocytes from patients with active disease were used as stimulators than when monocytes from patients with inactive disease were used. T4+ cells tested with autologous stimulatory monocytes harvested from an inactive stage of MS exhibited considerably higher proliferative responses than when stimulated with autologous monocytes obtained from an acute relapse. The decreased autologous proliferation of T4+ cells in MS patients was restored to normal levels after preincubation with ProT alpha in the environment of autologous monocytes. Our results demonstrate that ProT alpha is capable of fully restoring the deficient stimulatory function of MS monocytes and monocyte-associated functional defects of MS-derived T4+ cells.