OPTIMIZING PARENTAL SELECTION FOR GENETIC-LINKAGE MAPS
OPTIMIZING PARENTAL SELECTION FOR GENETIC-LINKAGE MAPS
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DOI：
10.1139/g93-024
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发表时间：
1993-02-01
期刊：
影响因子：
3.1
通讯作者：
SORRELLS, ME
中科院分区：
文献类型：
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作者：
ANDERSON, JA;CHURCHILL, GA;SORRELLS, ME
基于限制性片段长度多态性的遗传连锁图谱在许多方面都很有用；然而，不同的人群需要实现不同的目标。来自连锁图谱的克隆随后被探测到为特殊目的（如基因标记）而开发的群体中。因此，包含在初始图谱上的克隆必须在广泛的基因型上是多态的，以获得最大的效用。本研究的目的是(1)计算51个低拷贝DNA克隆的多态性信息含量值，(2)利用结果值选择潜在的定位亲本。通过对18个不同小麦基因型的限制性内切片段模式进行分类，利用基因多样性计算多态性信息含量。然后，通过检测多态克隆的比例，以及这些被映射的克隆在用于其他种群时产生多态性的可能性，对潜在亲本的组合进行比较。与来自最具多态性的潜在亲本的群体相比，鉴定出的基因型对将绘制出更多信息丰富的DNA克隆。用于鉴定克隆和对潜在亲本进行排序的方法也应适用于其他物种和标记类型。
Genetic linkage maps based on restriction fragment length polymorphisms are useful for many purposes; however, different populations are required to fulfill different objectives. Clones from the linkage map(s) are subsequently probed onto populations developed for special purposes such as gene tagging. Therefore, clones contained on the initial map(s) must be polymorphic on a wide range of genotypes to have maximum utility. The objectives of this research were to (i) calculate polymorphism information content values of 51 low-copy DNA clones and (ii) use the resulting values to choose potential mapping parents. Polymorphism information content was calculated using gene diversity by classifying restriction fragment patterns on a diverse set of 18 wheat genotypes. Combinations of potential parents were then compared by examining both the proportion of polymorphic clones and the likelihood that those mapped clones would give a polymorphism when used on other populations. Genotype pairs were identified that would map more highly informative DNA clones compared with a population derived from the most polymorphic potential parents. The methodologies used to characterize clones and rank potential parents should be applicable to other species and types of markers as well.