gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
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DOI：
10.3389/fgene.2018.00577
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发表时间：
2018-12-04
影响因子：
3.7
通讯作者：
Nie, Qinghua
中科院分区：
文献类型：
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作者：
Guo, Lijin;Huang, Weiling;Nie, Qinghua
非编码RNA在骨骼肌的生长和发育中发挥调节作用。我们之前的研究表明 gga-mir-133a-3p 是调节骨骼肌成肌细胞增殖和分化的潜在候选者。本研究的目的是揭示gga-mir-133a-3p在鸡成肌细胞增殖和分化中的调控机制。通过检测细胞增殖活性、细胞周期进程和EdU，我们发现gga-mir-133a-3p可以显着抑制成肌细胞的增殖。在成肌分化过程中，gga-mir-133a-3p上调，而gga-mir-133a-3p能显着促进分化相关肌源性因子上调，说明gga-mir-133a-3p能够促进成肌细胞的分化。转录水平和蛋白水平的验证证明gga-mir-133a-3p可以抑制PRRX1的表达，双荧光素酶实验也显示了它们的直接靶向关系。相应地，PRRX1能够显着促进成肌细胞增殖并抑制成肌细胞分化。在我们的研究中，我们证实gga-mir-133a-3p通过靶向PRRX1参与成肌细胞增殖和分化的调节。
Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mechanism of gga-mir-133a-3p in the proliferation and differentiation of chicken myoblasts. Through the detection of cell proliferation activity, cell cycle progression and EdU, we found that gga-mir-133a-3p can significantly inhibit the proliferation of myoblasts. In the process of myogenic differentiation, gga-mir-133a-3p is up-regulated, while gga-mir-133a-3p can significantly promote the up-regulation of differentiation-related muscle-derived factors, indicating that gga-mir-133a-3p can promote the differentiation of myoblasts. Validation at the transcriptional level and protein level proved that gga-mir-133a-3p can inhibit the expression of PRRX1, and the dual-luciferase assay also showed their direct targeting relationship. Correspondingly, PRRX1 can significantly promote myoblast proliferation and inhibit myoblast differentiation. In our study, we confirmed that gga-mir-133a-3p participates in the regulation of proliferation and differentiation of myoblasts by targeting PRRX1.