Characterization of interactions between and among components of the meiotic silencing by unpaired DNA machinery in Neurospora crassa using bimolecular fluorescence complementation
Characterization of interactions between and among components of the meiotic silencing by unpaired DNA machinery in Neurospora crassa using bimolecular fluorescence complementation
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DOI：
10.1534/genetics.107.079384
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发表时间：
2008-01-01
期刊：
影响因子：
3.3
通讯作者：
Shiu, Patrick K. T.
中科院分区：
文献类型：
--
作者：
Bardiya, Nirmala;Alexander, William G.;Shiu, Patrick K. T.
双分子荧光互补（BiFC）是基于黄色荧光蛋白（YFP）的两个非荧光片段之间的互补，当它们通过与它们共价连接的蛋白质之间的相互作用而结合时。我们已经成功地将BiFC应用于粗糙脉孢菌中，产生了两个参与受损DNA减数分裂沉默（MSUD）的基因，并观察到仅涉及SAD-1蛋白、仅SAD-2蛋白以及SAD-1和SAD-2蛋白的混合物的大分子复合物形成。
Bimolecular fluorescence complementation (BiFC) is based on the complementation between two non-fluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa rising two genes involved in meiotic silencing by impaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.